Summary
Copolymer beads from acrylonitrile and ethyl either butyl acrylate or vinyl acetate, with amino, hydroxyl or carboxyl groups were activated with glutaraldehyde or water-soluble carbodiimide and coupled to human serum albumin. The obtained immunoadsorbents were applied to the isolation of anti-human albumin anibody from rabbit antiserum. Among the 22 studied copolymers only a few showed satisfactory effectiveness for immunoaffinity chromatography, similar to that noted for immunoadsorbents from commerical polymers.
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References
I. Arvieux, A. F. Williams, in “Antibodies, A practical approach”,D. Catty, ed., IRL Press, Oxford, 1988, p. 113.
A. H. Chase, J. Biotechnol.1, 67 (1984).
J. L. Guesdon, S. Avrameas, J. Immunol. Methods11, 129 (1976).
N. A. L. Hurn, S. M. Chantlerl, Methods Enzymol.70A, 104 (1980).
S. Margel, H. Offarin, Anal. Biochem.128, 342 (1983).
J. R. Sportsman, G. S. Wilson, Anal. Chem.52, 2013 (1980).
A. Rapak, A. Szewczuk, B. N. Kolarz, A. Trochimczuk, M. Wojaczynska, Arch. Immunol. Ther. Exp.35, 811 (1987).
A. Wojaczynska, B. N. Kolarz, J. Chromatogr.358, 129 (1986).
B. Kolarz, J. Lobarzewski, A. Szewczuk, A. Rapak, A. Trochimczuk, M. Wojaczynska, J. Wierzchowski, Polish Patent No. 145,574 (1986).
A. Trochimczuk, B. Kolarz, “Reactive polymers”, 1989 (in press).
H. Hecker, J. Chromatogr.102, 135 (1974).
S. Siggia, “Quantitative Organic Analysis of Functional Groups”, John Wiley, New York, 1954, p. 9.
R. L. Gustafson, J. Phys. Chem.67, 2549 (1963).
R. G. Frost, J. F. Monthony, S. C. Eugelhorn, C. J. Siebert, Biochem. Biophys. Acta670, 163 (1981).
I. Miron, M. Wilchek, Appl. Biochem. Biochnol.11, 445 (1985).
A. M. Lew, J. Immunol. Methods72, 171 (1984).
O. H. Lowry, N. J. Rosenbrough, A. L. Farr, R. L. Rendall, J. Biol. Chem.193, 265 (1951).
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Rapak, A., Szewczuk, A., Kolarz, B. et al. The use of modified acrylic copolymers for the preparation of immunoadsorbents. Chromatographia 30, 428–431 (1990). https://doi.org/10.1007/BF02328511
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DOI: https://doi.org/10.1007/BF02328511