Summary
A fast and easy, iscratic high-performance liquid chromatographic method for the simultaneous determination of primidone, phenobarbital, phenytoin, carbamazepine and thiobutabarbital from serum samples has been developed. The optimal eluent composition is 36% acetonitrile, 24% methanol and 40% phosphate buffer. Using a programmable wavelength detector very low (sub-therapeutic) levels of the drugs can be measured.
This is a preview of subscription content, log in via an institution to check access.
References
J. T. Burke, J. P. Thénot, J. Chromatogr.,340, 199 (1985).
A. Nazareth, L. Jaramillo, B. L. Karger, R. W. Giese, L. R. Snyder, J. Chromatogr.309, 375 (1984).
S. H. Y. Wong, L. A. Buttes, A. C. Larsen, J. Liq. Chromatogr.11, 2039 (1988).
D. J. Gisch, B. Feibush, B. T. Hunter, T. L. Ascah, BioChromatography4, 206 (1989).
G. S. J. Hook, J. A. Ooms, J. P. Braamsa, LC-GC6, 1068 (1988).
L. E. Riad, R. J. Sawchuk, Clin. Chem.9, 1863 (1988).
S. Peaker, A. C. Mehta, S. Kumar, M. Feeley, J. Chromatogr.,497, 308 (1989).
A. M. Treston, W. D. Hooper, J. Chromatogr.,526, 59 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ohmacht, R., Matus, Z. & Soós, J. Rapid separation of some antiepileptic drugs in serum by HPLC. Chromatographia 32, 85–86 (1991). https://doi.org/10.1007/BF02262474
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02262474