Abstract
The immune response to adenoviral vectors can induce inflammation and loss of transgene expression in transfected tissues. This would limit the use of adenovirus-mediated gene transfer in disease states in which long-term gene expression is required. While studying the effect of the anti-adenoviral immune response in transplantation, we found that transgene expression persisted in cardiac isografts transfected with an adenovirus encoding β-galactosidase. Transfected grafts remained free of inflammation, despite the presence of an immune response to the vector. Thus, adenovirus-mediated gene transfer may have therapeutic value in cardiac transplantation and heart diseases. Furthermore, immunological limitations of adenoviral vectors for gene therapy are not universal for all tissue types.
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Acknowledgements
We thank G. Nabel and M. Imperiale for their comments regarding this manuscript. This work was supported by NIH grant R01 AI HL 31946.
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Chan, S., Li, K., Piccotti, J. et al. Tissue-specific consequences of the anti-adenoviral immune response: implications for cardiac transplants. Nat Med 5, 1143–1149 (1999). https://doi.org/10.1038/13467
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DOI: https://doi.org/10.1038/13467