Abstract
SEDIMENTATION in the ultracentrifuge has so far been followed quantitatively only by optical methods, depending upon the absorption or refraction of light by the substances studied. Even though they have been worked out to a high degree of accuracy, these methods for many important problems need to be supplemented by some procedure allowing chemical tests to be performed in the same run. For example, in a mixture of an enzyme or an antibody with its accompanying impurities, we do not know α priori the relationship between optical properties and the amount of active substance, and we cannot therefore definitely state which of the optically observed components in the mixture, if any, carries the activity. As the amount of substances of this kind in most cases can be defined only by an action measured by analytical methods, their sedimentation should be computed from analysis of samples taken out of the ultracentrifugal cell. In this laboratory1,2 and elsewhere3, several attempts have been made to take out samples of the ultracentrifuged solutions after concluding an experiment, but the arrangements have not been sufficiently perfect to allow quantitative application.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Eriksson-Quensel, I.-B., and Svedberg, T., Biol. Bull, 71, 498 (1936).
Heidelberger, M., Pedersen, K. O., and Tiselius. A., NATURE, 138, 165 (1936).
Wyckoff, B. W. G., Science, 84, 291 (1936).
Heidelberger, M., Sia, R. H. P., and Kendall, F. E., J. Exp. Med., 52, 477 (1930).
Biscoe, J., Herik, F., and Wyckoff, R. W. G., Science, 83, 602 (1936).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
TISELIUS, A., PEDERSEN, K. & SVEDBERG, T. Analytical Measurements of Ultracentrifugal Sedimentation. Nature 140, 848–849 (1937). https://doi.org/10.1038/140848a0
Issue Date:
DOI: https://doi.org/10.1038/140848a0
This article is cited by
-
Beitrag zur biologischen Bestimmung der Sedimentationskonstanten tierpathogener Viren in der „improvisierten“ Separationszelle
Kolloid-Zeitschrift (1957)
-
Specific Crystalline Protein and Nucleoprotein from a Plant Virus having Insect Vectors
Nature (1948)
-
�ber mikroskopische Beobachtungen an gereinigten Poliomyelitisviruspr�paraten
Archiv f�r die gesamte Virusforschung (1944)
-
A Simple Inexpensive Ultracentrifuge
Nature (1938)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.