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Nucleophagocytosis in Bone Marrow

Nature volume 196, pages 488–489 (1962)Cite this article

Abstract

INGESTED nuclear material has long been known within the reticular cells of normal lymphoid tissues and has led to the suggestion that the reticular cell may re-utilize lymphocytes and incorporate their deoxyribonucleic acid (DNA) into newly formed cells1,2. During autoradiographic studies of lymphocytes in the bone marrow, observations have been made on reticular cells including the ingestion of nuclear material labelled by the prior incorporation of tritium-labelled thymidine during DNA synthesis. Male Hartley guinea pigs weighing approximately 400 g were given a single, intraperitoneal dose of tritiated thymidine (1 µc./g body-weight: specific activity, 1.9 c./mM). After intervals of 25, 48 and 72 h humeral bone marrow was gently suspended in homologous serum and examined in smears. Auto-radiographs of smears were prepared by coating with melted Eastman Kodak NTB3 emulsion, exposing for 14 days and staining through the fixed emulsion with MacNeal's tetrachrome stain.

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Authors and Affiliations

  1. Department of Anatomy, University, Bristol,

    D. G. OSMOND

  2. Department of Anatomy, University of Washington, Seattle, Washington

    N. B. EVERETT

Authors
  1. D. G. OSMOND
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  2. N. B. EVERETT
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OSMOND, D., EVERETT, N. Nucleophagocytosis in Bone Marrow. Nature 196, 488–489 (1962). https://doi.org/10.1038/196488a0

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