Abstract
IT is well known that higher organisms can utilize the carbon atom of formate molecules for the synthesis of purines, as indeed can liver tissue in vitro. However, in rabbit and human bone marrows and in Ehrlich ascites cells of the mouse, formate-14C was found to be utilized very inefficiently for de novo synthesis of purines in vitro, but very efficiently for labelling thymine in deoxyribonucleic acid1–3. As liver extracts restore the ability of these cells to synthesize purines in vitro 4, it was suggested that, in vivo, the capacity of bone marrow cells for synthesis of purines may be so limited that they depend on the liver for the supply of purines. This inference was tested experimentally by measuring the amount of formate-14C incorporated into the deoxyribonucleic acid bases of bone marrow cells in control and hepatectomized rabbits.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Totter, J. R., J. Amer. Chem. Soc., 76, 2196 (1954).
Lajtha, L. G., Nature, 174, 1013 (1954).
Prusoff, W. H., Lajtha, L. G., and Welch, A. D., Biochim. Biophys. Acta, 20, 209 (1956).
Smellie, R. M. S., Thomson, R. Y., Goutier, R., and Davidson, J. N., Biochim. Biophys. Acta, 22, 585 (1956).
Lajtha, L. G., and Noyes, W. D., Exp. Cell Res. (in the press).
Lajtha, L. G., Oliver, R., and Ellis, F., Brit. J. Cancer, 8, 367 (1954).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
LAJTHA, L., VANE, J. Dependence of Bone Marrow Cells on the Liver for Purine Supply. Nature 182, 191–192 (1958). https://doi.org/10.1038/182191a0
Issue Date:
DOI: https://doi.org/10.1038/182191a0
This article is cited by
-
Induced pluripotent stem cells from subjects with Lesch-Nyhan disease
Scientific Reports (2021)
-
The E-NTPDase family of ectonucleotidases: Structure function relationships and pathophysiological significance
Purinergic Signalling (2006)
-
Changes in myocardial pyrimidine nucleotide levels following repeated injections of isoproterenol in rats
Pfl�gers Archiv European Journal of Physiology (1981)
-
Synthese von Purinnucleotiden in der menschlichen Leber und ihre Beeinflussung durch Benzbromaronum
Klinische Wochenschrift (1978)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.