Abstract
Granulocyte–macrophage colony stimulating factors (GM–CSFs) regulate the growth and differentiation of committed granulocyte and macrophage progenitor cells1–3. Operationally, the factors are recognized by their ability to stimulate the formation of granulocyte and macrophage colonies in semi-solid cultures of bone marrow cells. GM-CSFs are produced by a variety of tissues, and prominent cellular sources in man include T lymphocytes and mononuclear phagocytes1,4–7. The factors obtained from most sources are glycoproteins of molecular weight 20,000–70,000. At least two major GM-CSF subtypes have been distinguished in mice and humans on the basis of biological activity in vitro: one primarily stimulates the formation of macrophage colonies, while the other stimulates the fomation of both granulocyte and macrophage colonies1,8. We report here the translation and partial characterization of a messenger RNA for human GM-CSF that stimulates both granulocyte and macrophage colonies. The mRNA was isolated from a human T-lymphocyte cell line, and when injected into Xenopus laevis oocytes9 it directed the synthesis of biologically active GM-CSF.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Burgess, A. W. & Metcalf, D. Blood 56, 947–958 (1980).
Metcalf, D. Hemopoietic Colonies: In Vitro Cloning of Normal and Leukemic Cells (Springer, New York, 1977).
Moore, M. A. S. Clin. Hemat. 8, 287–310 (1979).
Chervenick, P. A. & LoBuglio, A. F. Science 178, 164–166 (1977).
Golde, D. W., Finley, T. N. & Cline, M. J. Lancet ii, 1397–1399 (1972).
Parker, J. W. & Metcalf, D. J. Immun. 122, 502–510 (1974).
Cline, M. J. & Golde, D. W. Nature 248, 703–770 (1974).
Stanley, E. R. Proc. natn. Acad. Sci. U.S.A. 76, 2969–2973 (1979).
Gurdon, J. B. The Control of Gene Expression in Animal Development (Harvard University Press, 1974).
Golde, D. W., Quan, S. G. & Cline, M. J. Blood 52, 1068–1072 (1978).
Lusis, A. J., Quon, D. H. & Golde, D. W. Blood 57, 13–21 (1981).
Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. & Rutter, W. I. Biochemistry 18, 5294–5299 (1979).
Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).
Bishop, J., Morton, J., Rosbash, M. & Richardson, M. Nature 250, 199–204 (1974).
Golde, D. W. & Cline, M. J. J. clin. Invest. 51, 2981–2983 (1972).
Messner, H. A., Till, J. E. & McCulloch, E. A. Blood 52, 701–710 (1973).
Pelham, H. R. B. & Jackson, R. J. Eur. J. Biochem. 67, 247–256 (1976).
Koeffler, H. P. & Golde, D. W. Science 200, 1153–1154 (1978).
Lusis, A. J. & Koeffler, H. P. Proc. natn. Acad. Sci. U.S.A. 77, 5346–5350 (1980).
Taniguchi, T., Pang, R. H., Yip, Y. K., Henriksen, D. & Vilcek, J. Proc. natn. Acad. Sci. U.S.A. 78, 3469–3472 (1981).
Nathan, I., Groopman, J. E., Quan, S. G., Bersch, N. & Golde, D. W. Nature 292, 842–844 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lusis, A., Golde, D., Quon, D. et al. Translation of mRNA for human granulocyte–macrophage colony stimulating factor. Nature 298, 75–77 (1982). https://doi.org/10.1038/298075a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/298075a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.