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Glucose-6-phosphatase Activity of Mouse Pancreatic Islets

Nature volume 219, pages 857–858 (1968)Cite this article

Abstract

THE rate of release of insulin by pancreatic islets is increased by glucose with a Km (for glucose) of approximately 10 mM (refs. 1 and 2). The rate of oxidation of glucose by mouse pancreatic islets is similarly dependent on glucose concentration and the Km (for glucose) is approximately 7 mM (ref. 3). Studies with mannoheptulose and phloridzin and measurements of islet glucose-6-phosphate concentration have indicated that glucose metabolism in pancreatic islets may be controlled by the rate of conversion of glucose to glucose-6-phosphate3,4. Glucose-6-phosphatase activity has been detected in rabbit islets5 and in the islets of obese-hyperglycaemic mice6, and we have previously noted activity in normal mouse islets in a study of hexokinase activity7. We have now investigated the possible role of glucose-6-phosphatase in the control of glucose phosphorylation in islets isolated from normal mouse pancreas by the collagenase method3.

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Authors and Affiliations

  1. Department of Biochemistry, University of Bristol Medical School,

    S. J. H. ASHCROFT & P. J. RANDLE

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  1. S. J. H. ASHCROFT
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  2. P. J. RANDLE
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ASHCROFT, S., RANDLE, P. Glucose-6-phosphatase Activity of Mouse Pancreatic Islets. Nature 219, 857–858 (1968). https://doi.org/10.1038/219857a0

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