Color pattern specific proteins in black scales in developing wings ofPrecis coenia Hübner (Nymphalidae, Lepidoptera)

Summary

A set of stage specific proteins of approximally 86 to 90 kDal are synthesized by isolated wings ofPrecis coenia on day 5 of the pupal stage. They are named “B proteins". They are synthesized in presumptive black wing areas in higher amounts than in presumptive white wing areas and are the major proteins synthesized on day 5. Wings from 5 days old pupae, which were incubated with³⁵S-methionine for 2 or 4 hours, incorporate radiolabel into presumptive black pattern elements. This is probably due to the localized synthesis of the above mentioned proteins. Injection of³⁵S-methionine into whole pupae on day 5 resulted in the labelling of the mature black and grey scales but not white scales. This radiolabel incorporation pattern corresponds exactly to the incorporation of the melanin precursor¹⁴C-tyrosine into the scales. The results indicate that the “B proteins” are specifically related to the formation of black and grey portions of thePrecis wing pattern. Injection of³⁵S-methionine into whole pupae on day 6 resulted in the labelling of the mature red scales probably due to labelling of “R proteins”, which may be involved in the formation of red pattern elements.