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Comparative study on hydrolysis of triolein in reversed micellar system catalyzed by different types of lipases

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Biotechnology Techniques

Abstract

Hydrolysis of triolein in AOT/isooctane reversed micelles by an sn-1,3-regioselective and a non-selective lipase were studied. Kinetics of the multistep reaction: decomposition of tri-, di- and monoacylglycerols and production of fatty acid were investigated separately. All the reactions was found to obey the Michaelis-Menten model and the apparent parameters (Michaelis-constants (Km) and maximal reaction rates (Vmax)) were determined both for non-selective and regioselective preparations.

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Authors and Affiliations

  1. Research Institute of Chemical Engineering of the Hungarian Academy of Sciences, Egyetem u. 2., 8200, Veszprém, Hungary

    K. Bélafi-Bakó, Á. Dombi, L. Szabó & E. Nagy

Authors
  1. K. Bélafi-Bakó
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  2. Á. Dombi
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  3. L. Szabó
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  4. E. Nagy
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Bélafi-Bakó, K., Dombi, Á., Szabó, L. et al. Comparative study on hydrolysis of triolein in reversed micellar system catalyzed by different types of lipases. Biotechnol Tech 9, 59–64 (1995). https://doi.org/10.1007/BF00153002

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  • DOI: https://doi.org/10.1007/BF00153002

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