Skip to main content
SpringerLink
Log in

Histamine release from human basophils by pepstatin A

  • Histamine and Kinins
  • Published:
Agents and Actions Aims and scope Submit manuscript

Abstract

Pepstatin A, a pentapeptide isolated from cultures of actinomycetes, induced histamine secretion from human basophils in the concentration range of 3×10−7 to 10−4 M. The characteristics of this reaction were similar to those of f-met-peptide-induced histamine release: pepstatin A-induced release required Ca2+ and the release reaction was complete within 2 min at 22 or 37°C, but did not occur at 4°C. Release by both pepstatin a and f-met-peptide was reversibly inhibited by two non-releasing analogs of f-met-peptide, CBZ-Phe-Met and BOC-Met-Leu-Phe, Further, there was complete cross-desensitization between pepstatin A and f-met-peptide, while cells desensitized to pepstatin A released normally with anti-IgE and vice versa. A variety of pharmacological agents had similar effects on both pepstatin A and f-met-peptide-induced release (e.g., no enhancement with D2O; marked enhancement with cytochalasin B). We suggest that pepstatin A induces histamine release from human basophils by activating a cell surface receptor(s), also activated by the synthetic tripeptide f-met-peptide.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

Anti-IgE:

rabbit anti-Fc∈

BOC-Met-Leu-Phe:

N-t-BOC-methionyl-l-leucyl-l-phenylalanine

CBZ-Phe-Met:

carbobenzoxy-phenylalanylmethionine

EDTA:

ethylenediaminetertraacetic acid

f-met-peptide:

formyl-methionyl-l-leucyl-l-phenylalanine

PIPES:

piperazine-N,N′-bis(2-ethanesulphonic acid)

P:

25 mM PIPES buffer, 110 mM NaCl, 5 mM KCl, pH 7.4

PC:

PIPES buffer containing 2.0 mM CaCl2.

References

  1. S.K. Ackerman andS.D. Douglas,Pepstatin A — a human leukocyte chemoattractant, Clin. Immunol. Immunopathol.14, 244–250 (1979).

    Article  PubMed  Google Scholar 

  2. E. Gillespie andL.M. Lichtenstein,Histamine release from human leukocytes: studies with deuterium oxide, colchicine and cytochalasin B, J. clin. Invest.51, 2941–2947 (1972).

    PubMed  Google Scholar 

  3. O. Kawanami, F. Basset, R. Barrios, J.G. Lacronique, V.J. Ferrans andR.G. Crystal,Hypersensitive pneumonitis in man. Light- and electron-microscopic studies of 18 lung biopsies, Am. J. Path.110, 275–289 (1983).

    PubMed  Google Scholar 

  4. G. Marone, A.K. Kagey-Sobotka andL.M. Lichtenstein,Effects of arachidonic acid and its metabolites on antigen-induced histamine release from human basophils in vitro, J. Immun.123, 1669–1677 (1979).

    PubMed  Google Scholar 

  5. G. Marone, S. Poto, R. Petracca, M. Triggiani, E. de Lutio di Castelguidone andM. Condorelli,Activation of human basophils by staphylococcal protein A. 1. The role of cyclic AMP, arachidonic acid metabolites, microtubules and microfilaments, Clin. exp. Immunol.50, 661–668 (1982).

    PubMed  Google Scholar 

  6. G. Marone, M. Columbo, S. Poto andM. Condorelli,Inhibition of histamine release from human basophils in vitro by calmodulin antagonists, Clin. Immunol. Immunopathol.28, 334–340 (1983).

    Article  PubMed  Google Scholar 

  7. G. Marone, G. Ambrosio, D. Bonaduce, A. Genovese, M. Triggiani andM. Condorelli,Inhibition of IgE-mediated histamine release from human basophils and mast cells by fenoterol, Int. Archs Allergy appl. Immun.74, 356–361 (1984).

    Google Scholar 

  8. R.D. Nelson, S.K. Ackerman, V.D. Fiegel, M.P. Bauman andS.D. Douglas,Cytotaxin receptors of neutrophils: evidence that F-methionyl peptides and pepstatin share a common receptor, Infect. Immun.26, 996–999 (1979).

    PubMed  Google Scholar 

  9. J.T. O'Flaherty, H.J. Showell, D.L. Kreutzer, P.A. Ward andE.L. Becker,Inhibition of in vivo and in vitro neutrophil responses to chemotactic factors by a competitive antagonist, J. Immun.120, 1326–1332 (1978).

    PubMed  Google Scholar 

  10. R. Patterson, M. Roberts, R.C. Roberts, D.A. Emanuel andJ.N. Fink,Antibodies of different immunoglobulin classes against antigens causing famer's lung, Am. Rev. resp. Dis.114, 315–324 (1976).

    PubMed  Google Scholar 

  11. M. Schmitt, R.G. Painter, A.J. Jesaitis, K. Preissner, L.A. Sklar andC.G. Cochrane,Photoaffinity labeling of the N-formyl peptide receptor binding site of intact human polymorphonuclear leukocytes. A label suitable for following the fate of the receptorligand complex. J. biol. Chem.258, 649–654 (1983).

    PubMed  Google Scholar 

  12. R.P. Siraganian andW.A. Hook,Mechanism of histamine release by formyl methonine-containing peptides, J. Immun.119, 2078–2083 (1977).

    PubMed  Google Scholar 

  13. R.J. Smith, B.J. Bowman andS.S. Iden,Pepstatin A-induced degranulation and superoxide anion generation by human neutrophils, Clin. Immunol. Immunopathol.22, 83–93 (1982).

    Article  PubMed  Google Scholar 

  14. H. Umezawa, T. Aoyagi, H. Morishima, M. Matsuzaki, M. Hamada andT. Takeuchi,Pepstatin, a new pepsin inhibitor produced by actinomycetes, J. Antibiotics23, 259–262 (1970).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medicine, University of Naples II School of Medicine, Via S. Pansini 5, 80131, Naples, Italy

    Gianni Marone, Michele Columbo, Laura Soppelsa & Mario Condorelli

Authors
  1. Gianni Marone
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Michele Columbo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Laura Soppelsa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mario Condorelli
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Marone, G., Columbo, M., Soppelsa, L. et al. Histamine release from human basophils by pepstatin A. Agents and Actions 16, 306–309 (1985). https://doi.org/10.1007/BF01982863

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01982863

Keywords

Search

Navigation