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Inhibition of histamine release from rat peritoneal mast cells by a factor from human serum — identification as transferrin

  • Histamine and Kinins
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Abstract

Recently, we described the presence of a blocking factor (BF) in rat serum which inhibited the histamine release from rat mast cellsin vivo andin vitro. The blocking activity was demonstrated in human serum as well. Purification of the human-BF was carried out in a similar way as previously described for the rodent molecule. Both protein fractions produced a marked suppression of histamine release from rat mast cells and human basophils in a dose-dependent fashion.

Qualitative analysis of the purified preparations demonstrated a major component with a molecular weight of 70 000 daltons. In human serum the blocking factor was identified as transferrin by serological and biochemical methods. It is suggested that this molecule may play an important role in regulating histamine release during allergic and inflammatory reactions.

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Abbreviations

BBS:

borate buffered saline

BF:

blocking factor

BSA:

bovine serum albumin

DEAE:

diethyl-amino-ethyl-cellulose

DIP:

Dactylis inhibitory protein

IA:

immunoaffinity

IEP:

isoelectric point

kd:

kilodalton

LMB:

lymphocytes-monocytes-basophils

N. b.:

Nippostrongylus brasiliensis

n.d.:

not done

PCA:

passive cutaneous anaphylaxis

RPC:

rat peritoneal exudate cells

SDS:

sodium dodecyl sulphate

TCM:

Tris, Ca++, Mg++ buffer

Tris:

Trishydroxy-methyl-amino-methane

References

  1. R. Heatly,The gastrointestinal mast cell, Scand. J. Gastroenterol.18, 449–453 (1983).

    Google Scholar 

  2. J. Bienenstock, A. Befus, F. Pearce, J. Denburg and R. Goodacre,Mast cell heterogeneity: derivation and function with emphasis on the intestine, J. Allergy Clin. Immunol.70, 407–412 (1982).

    Google Scholar 

  3. T. Ishizaka and K. Ishizaka,Activation of mast cells for mediator release through IgE-receptors. InProg. Allergy Vol. 34, pp. 188–235 (Ed. K. Ishizaka), S. Karger, Basel 1984.

    Google Scholar 

  4. R. P. Schleimer, L. M. Lichtenstein and E. Gillespie,Inhibition of basophil histamine release by anti-inflammatory steroids, Nature, London292, 454–455 (1981).

    Google Scholar 

  5. D. Lagunoff, T. Martin and G. Read,Agents that release histamine from mast cells, Ann. Rev. Pharmacol. Toxicol.23, 331–351 (1983).

    Google Scholar 

  6. G. Marone, G. Ambrosio, D. Bonaduce, A. Genovese, M. Triggiani and M. Condorelli,Inhibition of IgE-mediated histamine release from human basophils and mast cells by fenoterol, Int. Archs. Allergy appl. Immunol.74, 356–361 (1984).

    Google Scholar 

  7. S. T. Holgate, R. A. Lewis and K. F. Austen,The role of cyclic nucleotides in mast cell activation and secretion, In:Fougerau, Dausset, Progress in ImmunologyIV, 846–859 (1980).

  8. F. Hirata, W. J. Strittmatter and J. Axelrod, β-adrenergic receptor agonists increase phospholipid methylation, membrane fluidity and β-adrenergic receptor-adenylate cyclase coupling, Proc. Natl. Acad. Sci. USA76.1, 368–372 (1979).

    Google Scholar 

  9. B. Schick, K. F. Austen and L. Schwartz,Activation of rat serosal mast cells by chymase, an endogenous secretory granule protease, J. Immunol.132, 2571–2577 (1984).

    Google Scholar 

  10. T. Ishizaka, D. Conrad, E. Schulman, A. Sterk and K. Ishizaka,Biochemical analysis of initial triggering events of IgE-mediated histamine release from human lung mast cells, J. Immunol.130, 2357–2362 (1983).

    Google Scholar 

  11. D. MacGlashan, R. Schleimer, S. Peters, E. Schulman, K. Adams, A. Sobotka, H. Newball and L. Lichtenstein,Comparative studies of human basophils and mast cells, Fed. Proc.42, 2504–2509 (1983).

    Google Scholar 

  12. T. Ishizaka, A. R. Sterk, M. Daeron, E. L. Becker and K. Ishizaka,Biochemical analysis of desensitization of mouse mast cells, J. Immunol.135, 492–501 (1985).

    Google Scholar 

  13. W. König, P. Pfeiffer and H. W. Kunau,Effect of arachidonic acid metabolites on the histamine release from human basophils and rat mast cells, Int. Arch. Allergy appl. Immunol. suppl.1, 66, 149–151 (1981).

    Google Scholar 

  14. D. L. Marquardt C. W. Parker and T. J. Sullivan,Potentiation of mast cell mediator release by adenosine, J. Immunol.120, 871–878 (1978).

    Google Scholar 

  15. W. König and H. W. Henn,On the latency period of sensitization. Inhibition of passive cutaneous anaphylaxis by a factor in normal rat serum, Int. Archs. Allergy appl. Immunol.58, 182–194 (1979).

    Google Scholar 

  16. K. Theobald and W. König,Characterization of a factor from rat serum with blocking activity in the passive cutaneous anaphylactic reaction, Int. Archs. Aldlergy appl. Immunol.70, 1–11 (1983).

    Google Scholar 

  17. S. Mecheri, G. Peltre and B. David,Induction of a passive cutaneous anaphylaxis inhibitory factor in mice, Int. Archs. Allergy appl. Immunol.78, 290–299 (1985).

    Google Scholar 

  18. B. Ogilvie,Reagin-like antibodies in rats infected with the nematode parasite Nippostrongylus brasiliensis, Immunology12, 113–131 (1967).

    Google Scholar 

  19. W. König, M. Baron and F. Ehring,Effect of calcium mineral solutions on the histamine release from human peripheral leukocytes, Drug Res.34, 52–54 (1984).

    Google Scholar 

  20. O. Lowry, N. Rosebrough, A. Farr and R. Randall,Protein measurement with the Folin phenol reagent, J. Biol. Chem.193, 265–275 (1951).

    Google Scholar 

  21. B. Lughtenberg, J. Meijers, R. Peters, P. van der Hoek and L. van Alphen,Electrophoretic resolution of the major outer membrane protein of E. coli K12 into four major bands, FEBS-Letters58, 254–258 (1975).

    Google Scholar 

  22. J. Scheidegger,Une micro-methode de l'immuno-electrophorese, Int. Archs. Allergy appl. Immunol.7, 103–110 (1955).

    Google Scholar 

  23. S. March, I. Parikh and P. Cuatrecasas,A simplified method for cyanogen bromide activation of agarose for affinity chromatography, Anal. Biochem.60, 149–152 (1974).

    Google Scholar 

  24. K. Theobald, W. Gross-Weege, J. Keymling and W. König,Inhibition of histamine release in vitro by a blocking factor from human serum: Comparison with the iron binding proteins transferrin and lactoferrin, Agents and Actions, submitted.

  25. A. Böyum,A one-stage procedure for isolation of granulocytes and lymphocytes from human blood. General sedimentation properties of white blood cells in a 1×g gravity field, Scan. J. Clin. Invest.97, 77–83 (1968).

    Google Scholar 

  26. K. Heide and H. G. Schwick,Salt fractionation of immunoglobulins, InHandbook of Experimental Immunology, Vol. 1 (Ed. D. M. Weir), 7.1–7.11 (1978).

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Authors and Affiliations

  1. Lehrstuhl für Medizinische Mikrobiologie und Immunologie-Arbeitsgruppe für Infektabwehrmechanismen, RUHR-Universität Bochum, Universitätsstr. 150, Postfach 10 21 48, D-4630, Bochum, West Germany

    W. Groß-Weege, K. Theoblad & W. König

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  1. W. Groß-Weege
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  2. K. Theoblad
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  3. W. König
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Additional information

In partial fulfilment of W. G.-W.'s M.D.-thesis.

Supported by Deutsche Forschungsgemeinschaft (DFG), Kö 427/7-2.

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Groß-Weege, W., Theoblad, K. & König, W. Inhibition of histamine release from rat peritoneal mast cells by a factor from human serum — identification as transferrin. Agents and Actions 19, 10–17 (1986). https://doi.org/10.1007/BF01977250

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  • DOI: https://doi.org/10.1007/BF01977250

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