Abstract
A novel and very useful technique was developed to assay a lysosomal enzyme released into the joint synovial fluid during zymosan-induced arthritis in mice.
This β-glucuronidase activity correlated with histomorphological changes observed in the joint: vasculitis, synovitis and pannus formation.
The antiarthritic drug triamcinolone acetonide improved both biochemical and histological parameters.
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E. C. Keystone, H. U. Schorlemmer, C. Pope and A. C. Allison,Zymosan-induced arthritis. A model of chronic proliferative arthritis following activation of the alternative pathway of complement. Arthritis Rheum.20, 1396–1401 (1977).
H. U. Schorlemmer, P. Davies, W. Hylton, M. Gugic and A. C. Allison,The selective release of lysosomal acid hydrolases from mouse peritoneal macrophages by stimuli of chronic inflammation. Br. J. Exp. Path.58, 315–326 (1977).
H. U. Schorlemmer, D. Bieter and A. C. Allison,Complement activation by the alternative pathway and macrophage enzyme secretion in the pathogenesis of chronic inflammation. Immunology32, 929–940 (1977).
M. B. Goldlust and L. C. Rich,Chronic immune synovitis in rabbits. I. Immunoglobulin and β-glucuronidase analysis of synovial tissues and joint exudates. Agents and Actions11, 723–728 (1981).
M. B. Goldlust and L. C. Rich,Chronic Immune synovitis in rabbits. II. Modulation by anti-inflammatory and antirheumatic agents. Agents and Actions.11, 729–735 (1981).
P. S. Ringrose, M. A. Parr and M. McLaren,Effects of antiinflammatory and other compounds on the release of lysosomal enzymes from macrophages. Biochemical Pharmacology24, 607–614 (1975).
D. Brackertz, G. F. Mitchell and I. R. Mackay,Antigen-induced arthritis in mice. I. Induction of arthritis in various strains of mice. Arthritis Rheum.20, 841–850 (1977).
D. Brackertz, G. F. Mitchell, M. A. Vadas, I. R. Mackay and J. F. Miller,Studies on antigen-induced arthritis in mice. II. Immunologic correlates of arthritis susceptibility in mice. J. Immunol.118, 1639–1648 (1977).
D. Brackertz, G. F. Mitchell, M. A. Vadas and I. R. Mackay,Studies on antigen-induced arthritis in mice. III. Cell and serum transfer experiments. J. Immunol.118, 1645–1654 (1977).
A. N. Theofilopoulos and F. J. Dixon,Etiopathogenesis of murine SLE. Immunol. Rev.55, 179–183 (1981).
L. Hang, A. N. Theofilopoulos and F. Dixon,A spontaneous rheumatoid arthritis like disease in MRL/l mice. J. Exp. Med.115, 1690–1701 (1982).
A. N. Theofilopoulos R. S. Balderas, L. H. Hang and F. J. Dixon,Monoclonal IgM Rheumatoid Factors derived from arthritic MRL/Mp-Ipr mice. J. Exp. Med.158, 901–919 (1983).
F. O'Sullivan, H. G. Fassbender, S. Gay and W. Koopman,Etiopathogenesis of the rheumatoid arthritis-like disease in MRL/l mice. I. The histomorphologic basis of joint destruction. Arthritis Rheum.28, 529–536 (1985).
M. W. Kruijsen, W. B. van den Berg, L. B. van de Putte and W. J. van den Broek,Detection and quantification of experimental joint inflammation in mice by measurement of 99 m Tc-pertechnetate uptake. Agents and Actions11, 640–642 (1981).
R. Luukkainen, R. Talonen, E. Uksila and K. Auerma,Relationship between some joint fluid, blood and serum variables and radiological destruction in knee joints in arthritic patients. Scand. J. Rheumatology15, 399–406 (1986).
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Folliard, F., Terlain, B. A novel method for the sampling of synovial fluid in mice. Assay of a synovial lysosomal enzyme in zymosan-induced arthritis. Agents and Actions 25, 139–145 (1988). https://doi.org/10.1007/BF01969105
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DOI: https://doi.org/10.1007/BF01969105