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Effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on substance P-induced histamine release from rat peritoneal mast cells

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Abstract

12-O-tetradecanoylphorbol-13-acetate (TPA, 1 to 30 ng/ml) produced a dose-related inhibition of substance P (SP)-induced histamine release from rat peritoneal mast cells. TPA itself induced some histamine release over this concentration range (maximum release about 20% of total). Maximum inhibition of SP-induced release by TPA required preincubation with TPA for at least 10 min. The inhibitory action of TPA was observed in the absence as well as in the presence of extracellular calcium (0.4 mM). Inhibition of diacylglycerol kinase by R 59022 or of diacylglycerol lipase by RHC 80267 reduced SP-induced histamine release. Oleolylacetylglycerol (OAG, 50 μM) inhibited histamine release induced by SP but was less potent than TPA. It is concluded that protein kinase C activation in rat peritoneal mast cells is associated with inhibition of SP-induced histamine release.

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References

  1. J. C. Foreman, J. L. Mongar and B. D. Gomperts,Calcium ionophores and movement of calcium ions following the physiological stimulus to a secretory process. Nature245, 249–251 (1973).

    PubMed  Google Scholar 

  2. J. C. Foreman, M. B. Hallett and J. L. Mongar,The relationship between histamine secretion and 45 calcium uptake by mast cells. J. Physiol.271, 193–214 (1977).

    PubMed  Google Scholar 

  3. M. Ennis, F. L. Pearce, A. Truneh and J. R. White,Role of intra-and extracellular calcium in histamine release from rat peritoneal mast cells. Agents and Actions11, 51–54 (1981).

    PubMed  Google Scholar 

  4. Y. Katakami, K. Kaibuchi, M. Sawamura, Y. Takai and Y. Nishizuka,Synergistic action of protein kinase C and calcium for histamine release from rat peritoneal mast cells. Biochem. Biophys. Res. Commun.121, 573–578 (1984).

    PubMed  Google Scholar 

  5. M. E. Cantwell and J. C. Foreman,Characteristics of the effect of phorbol ester on rat mast cells: interaction with anti-IgE. Agents and Actions18, 77–80 (1986).

    PubMed  Google Scholar 

  6. J. C. Foreman and J. L. Mongar,The role of alkaline earth ions in anaphylactic histamine secretion. J. Physiol.224, 753–769 (1972).

    PubMed  Google Scholar 

  7. C. M. S. Fewtrell, J. C. Foreman, C. C. Jordan, P. Oehme, H. Renner and J. M. Stewart,The effects of substance P on histamine and 5-hydroxytryptamine release in the rat. J. Physiol.330, 393–411 (1982).

    PubMed  Google Scholar 

  8. D. De Chaffoy de Courcelles, P. Roevens and H. van Belle,R 59022, a diacylglycerol kinase inhibitor. J. biol. Chem.260, 15762–15770 (1985).

    PubMed  Google Scholar 

  9. C. A. Sutherland and D. Amin,Relative activities of rat and dog platelet phospholipase A 2 and diglyceride lipase. J. biol. Chem.257, 14006–14010 (1982).

    PubMed  Google Scholar 

  10. R. Sagi-Eisenberg, H. Lieman and I. Pecht,Protein kinase C regulation of the receptor-coupled calcium signal in histaminesecreting rat basophilic leukaemia cells. Nature313, 59–60 (1985).

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology, University College London, Gower Street, WC1E 6BT, London, UK

    J. McLeod, N. A. Hayes &  J. C. Foreman

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  1. J. McLeod
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  2. N. A. Hayes
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  3. J. C. Foreman
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McLeod, J., Hayes, N.A. & Foreman, J.C. Effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on substance P-induced histamine release from rat peritoneal mast cells. Agents and Actions 30, 140–142 (1990). https://doi.org/10.1007/BF01969021

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