Skip to main content
SpringerLink
Log in

A toxic substance from the sea urchinToxopneustes pileolus induces histamine release from rat peritoneal mast cells

  • Allergy, Histamine and Kinins
  • Published:
Agents and Actions Aims and scope Submit manuscript

Abstract

A toxic substance (P-II fraction), fractionated from the pedicellariae of the sea urchinToxopneustes pileulus, dose-dependently caused the histamine release from rat peritoneal mast cells. The histamine release induced by P-II fraction increased with time, while compound 48/80 caused a more rapid histamine release. The dose-response curve for P-II fraction was studied with concentration 0.03–2.0 mg/ml. This reaction was dependent on Ca2+ and temperature. When glucose (5.5. mM) was omitted during the incubation step, the histamine release induced by P-II fraction was significantly reduced as compared to that of compound 48/80. Pyruvate reversed this reduction. On the other hand, the histamine release induced by P-II fraction was effectively potentiated by the addition of glucose (11.0 mM), but not that by compound 48/80. These results suggest that P-II fraction-induced histamine release differs from that of compound 48/80 disregards to the effects of glucose, because this histamine release appears to be more sensitive to the glycolytic pathway than compound 48/80-induced histamine release.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. B. W. Halstead,Poisonous and Venomous Marine Animals of the World (2nd Ed.). pp. 187–212, Dorwin Press, Inc. New Jersey (1988).

    Google Scholar 

  2. T. Fujiwara,On the poisonous pedicellaria of Toxopneustes pileolus. Annotnes zool. Jap.15, 62–69 (1935).

    Google Scholar 

  3. C. B. Alender, G. A. Feigen and J. T. Tomita,Isolation and characterization of sea urchin toxin. Toxicon3, 9–17 (1965).

    PubMed  Google Scholar 

  4. A. Kimura, H. Hayashi and M. Kuramoto,Studies of sea urchi-toxins; Separation, purification and pharmacological actions of toxinic substances. Jap. J. Pharmac.25, 109–120 (1975).

    Google Scholar 

  5. E. G. Mendes, L. Abbud and S. Umiji,Cholinergic action of homogenates of sea urchin pedicellariae. Science139, 408–409 (1953).

    Google Scholar 

  6. G. A. Feigen, E. Sanz and C. B. Alender,Studies on the mode of action of sea urchin toxin-1. Conditions affecting release of histamine and other agents from isolated tissues. Toxicon.4, 161–175 (1966).

    Article  PubMed  Google Scholar 

  7. G. A. Feigen, L. Hadji, R. A. Pfeffer and G. Markus,Studies on the mode of atack of sea urchin toxin on natural and synthetic substrates. 1. Conditions determining the formation and isolation of plasma kinins. Physiol. Chem. & Physics.2, 309–322 (1970).

    Google Scholar 

  8. A. Kimura and H. Nakagawa,Action of an extract from the sea urchin Toxopneustes pileolus on isolated smooth muscle. Toxicon.18, 689–693 (1980).

    Article  PubMed  Google Scholar 

  9. H. Nakagawa, A. Kimura, M. Takei and K. Endo,Histamine release from rat mast cells induced by an extract from the sea urchin Toxopneustes pileolus. Toxicon.20, 1095–1097 (1982).

    Article  PubMed  Google Scholar 

  10. A. Kumura, H. Nakagawa, H. Hayashi and K. Endo,Seasonal changes in contractile activity of a toxic substance from the pedicellaria of the sea urchin Toxopneustes pileolus. Toxicon.23, 353–358 (1984).

    Article  Google Scholar 

  11. M. Takei, T. Matumoto, K. Endo and M. Muramatu,Inhibitory effect of anti-allergic agent NCO-650 on histamine release induced by various secretagogues. Agents and Actions35, 17–21 (1988).

    Google Scholar 

  12. A. Németh and P. Röhlich,Rapid Separation of rat peritoneal mast cells with Percoll. Eur. J. Cell Biol.20, 272–275 (1980).

    PubMed  Google Scholar 

  13. P. A. Shore, A. Burkhalter and V. H. Cohn,A method for the fluorometric assay of histamine in tissue. J. Pharmac. Exp. Ther.127, 182–186 (1959).

    Google Scholar 

  14. J. L. Mongar and H. O. Schild,Effect of temperature on the anaphylactic reaction. J. Physiol.135, 320–338 (1957).

    PubMed  Google Scholar 

  15. F. L. Pearce,Calcium and histamine secretion from mast cells. Prog. Med. Chem.19, 59–109 (1982).

    PubMed  Google Scholar 

  16. F. L. Pearce,Biochemical events involved in the release of anaphylactic mediators from mast cells. Asthma Reviews1, 95–139 (1987).

    Google Scholar 

  17. G. S. Chhatwal, G. Ahnert-Hilger, L. Beress and E. Habermann,Palytoxin both induces and inhibits the release of histamine from rat mast cells. Int. Archs. Allergy. appl. Immun.68, 97–100 (1982).

    Google Scholar 

  18. L. G. Garland and T. Johansen,The relationship between energy metabolism and the action of inhibitory of histamine release. Br. J. Pharmac.61, 237–242 (1977).

    Google Scholar 

  19. T. Johansen and N. Chakravarty,Dependence of histamine release from rat mast cells on adenosine triphosphate. Naunyn-Schmiedebergs Arch. Pharmac.275, 457–463 (1972).

    Article  Google Scholar 

  20. T. Johansen and N. Chakravaty,The utilization of adenosine triphosphate in rat mast cells during histamine release induced by anaphylactic reaction and compound 48/80. Naunyn-Schmiedebergs Arch. Pharmac.288, 243–260 (1975).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacology, School of Pharmacy, Tokushima Bunri University, 770, Tokushima, Japan

    M. Takei & K. Endo

  2. Department of Health Science, Faculty of Integrated Arts and Sciences, University of Tokushima, 770, Tokushima, Japan

    H. Nakagawa & A. Kimura

Authors
  1. M. Takei
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Nakagawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Kimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Endo
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Takei, M., Nakagawa, H., Kimura, A. et al. A toxic substance from the sea urchinToxopneustes pileolus induces histamine release from rat peritoneal mast cells. Agents and Actions 32, 224–228 (1991). https://doi.org/10.1007/BF01980878

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01980878

Keywords

Search

Navigation