Abstract
T lymphocytes present in allergically inflamed tissue synthesize and secrete the cytokines IL-3, IL-4, IL-5 and GM-CSF which may act as chemotaxins on eosinophils. In contrast to the former cytokines, IL-4 is chemotactic only for eosinophils from peripheral blood of patients with atopic dermatitis and not for eosinophils from normal individuals. IL-4 has the same chemotactic potency as the other cytokines. The optimal chemotactic potency is reached at a concentration of 10 nM. In contrast, neutrophils do not respond chemotactically to IL-4. Checkerboard analysis, inhibition studies with monoclonal anti-IL-4. Abs and desensitization experiments indicated specific interaction of IL-4 with eosinophils. In eosinophils from normal individuals, IL-4 responsiveness could be induced by pretreatment of the cells with IL-5 and GM-CSF. In addition to the fact that IL-4 may be responsible for selective eosinophil transendothelial migration, IL-4 may exert an important modulatory mode of action on eosinophil migration and function within allergically inflamed tissue. Our findings suggest the presence of a functional IL-4R on eosinophils from atopic dermatitis patients.
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References
C. Walker, J-C. Virchow Jr., P. L. B. Bruijnzeel and K. Blaser,T-cell subsets and their soluble products regulate eosinophilia in allergic and non-allergic asthma. J. Immunol.146, 1829–1836 (1991).
T. Kishimoto (Ed),Interleukins: Molecular Biology and Immunology. Chem Immunol. Vol. 51, pp. 107–134, Basel, Karger 1992.
P. L. B. Bruijnzeel, P. H. M. Kuijper, S. Rihs, S. Betz, R. A. J. Warringa and L. Koenderman,Eosinophil migration in atopic dermatitis I: Increased migratory response to N-formyl-methionyl-leucyl-phenyalanine, neutrophil-activating factor, platelet-activating factor and platelet factor 4. J. Invest. Dermatol.100, 137–142 (1993).
T. T. Hansel, J. D. Pound, D. Pilling, G. D. Kitas, M. Salmon, T. A. Gentle, S. S. Lee and R. A. Thompson,Purification of human blood eosinophils by negative selection using immunomagnetic beads. J. Immunol. Methods122, 97–103 (1989).
R. Moser, J. Fehr and P. L. B. Bruijnzeel,Interleukin-4 controls the selective extravasation of eosinophils from allergic individuals. J. Immunol.149, 1432–1438 (1992).
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Dubois, G.R., Bruijnzeel-Koomen, C.A.F.M. & Bruijnzeel, P.L.B. IL-4-induced migration of eosinophils in allergic inflammation. Agents and Actions 41 (Suppl 2), C256–C257 (1994). https://doi.org/10.1007/BF01987660
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DOI: https://doi.org/10.1007/BF01987660