Abstract
The direct protective action of adenosine and prostaglandin E2 (PGE2) was examined in an isolated gastric gland preparation in rabbits. Ethanol, (8%, v/v) incubation markedly increased the release of lactate dehydrogenase (LDH) and number of non-viable glands in the preparation. Both effects were prevented by PGE2 preincubation in a concentration (10−6, 1.4×10−5 or 2.8×10−5 M)-dependent manner. The protective action was smaller in adenosine-treated groups, and yet the highest concentration (10−4 M) of the compound also significantly inhibited the cytotoxic effects of ethanol. These findings indicate that both adenosine and PGE2 possess cytoprotective action on gastric glands in rabbits, but the former compound exerts its action beyond physiological concentrations. It is concluded that endogenous PGE2, but not adenosine may act as an ulcer modulator in the stomach.
Similar content being viewed by others
References
C. H. Cho, N. X. Liao, X. G. Liu, W. M. Hui, C. T. Luk and S. K. Lam,Effect of adenosine on gastric and cardiovascular systems, and ethanol ulcerations in male and female rats. Eur. J. Pharmacol.201, 157–162 (1991).
C. H. Cho and C. W. Ogle,The modulatory action of adenosine on gastric function and ethanol-induced mucosal damage in rats. Dig. Dis. Sci.35, 1334–1339 (1990).
J. G. Gerber, S. Fadul, N. A. Payne and A. S. Nies,Adenosine: A modulator of gastric acid secretion in vivo. J. Pharmacol. Exp. Ther.231, 109–113 (1984).
S. J. Konturek, A. Robert, A. J. Hanchar and J. E. Nezamis,Comparison of prostacycline and prostaglandin E 2 on gastric secretion, gastrin release, and mucosal blood flow in dogs. Dig. Dis. Sci.25, 673–679 (1980).
A. Robert,Antisecretory, antiulcer, cytoprotective and diarrheogenic properties of prostaglandins. Adv. Prostagl. Thromboxane Res.2, 507–520 (1976).
T. K. Chaudhury and A. Robert,Prevention by mild irritants of gastric necrosis produced in rats by sodium taurocholate. Dig. Dis. Sci.25, 830–836 (1980).
A. Robert,Cytoprotection by prostaglandins. Gastroenterology77, 761–767 (1979).
A. Robert, J. E. Nezamis, C. Lancaster and A. J. Hanchar,Cytoprotection by prostaglandins in rat. Prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic NaCl and thermal injury. Gastroenterology77, 433–443 (1979).
E. R. Lacy and S. Ito,Microscopic analysis of ETOH damage to rat gastric mucosa after treatment with a prostaglandin. Gastroenterology83, 619–625 (1982).
P. H. Guth, G. Paulsen and H. Nagata,Histologic and microcirculatory changes in alcohol-induced gastric lesions in the rats: Effects of prostaglandin cytoprotection. Gastroenterology87, 1083–1090 (1984).
K. L. Schmidt, J. M. Henagan, G. S. Smith, P. J. Hilburn and T. A. Miller,Prostaglandin cytoprotection against ETOH-induced gastric injury in the rat. A histologic and cytologic study. Gastroenterology88, 649–659 (1985).
T. Arakawa, H. Satoh, A. Nakamura, H. Nebiki, T. Fukuda, H. Sakuma, H. Nakamura, M. Ishikawa, M. Siki and K. Kobayashi,Effects of zinc l-carnosine on gastric mucosal and cell damage caused by ETOH in rats. Dig. Dis. Sci.35, 559–566 (1990).
C. H. Cho, W. M. Hui, B. W. Chen, C. T. Luk and S. K. Lam,The cytoprotective effects of zinc l-carnosine on ethanol-induced gastric gland damage in rabbits. J. Pharm., Pharmacol.44, 364–365 (1992).
M. Chvapil,New aspects in the biological role of zinc: A stabilizer of macromolecules and biological membranes. Life Sci.13, 1041–1049 (1973).
J. D. Geiger and G. B. Glavin,Adenosine receptor activation in brain reduces stress-induced ulcer formation. Eur. J. Pharmacol.115, 185–190 (1985).
V. S. Westerberg and J. D. Geiger,Central effects of adenosine analogs on stress-induced gastric ulcer formation. Life Sci.41, 2201–2205 (1987).
S. J. Konturek and A. Robert,Cytoprotection of canine gastric mucosa by prostacyclin: Possible mediation by increased mucosal blood flow. Digestion25, 155–163 (1982).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cho, C.H., Chen, B.W., Luk, C.T. et al. The gastric cytoprotective action of adenosine and prostaglandin E2 in rabbits. Agents and Actions 42, 146–148 (1994). https://doi.org/10.1007/BF01983481
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01983481