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Increased migration of neutrophils to granulocyte-colony stimulating factor in rat carrageenin-induced pleurisy: Roles of complement, bradykinin, and inducible cyclooxygenase-2

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Abstract

Administration of human recombinant granulocyte colony-stimulating factor (G-CSF, 100 μg/kg/day, s.c) to rats for 4 days significantly increased circulating neutrophil counts (by 1130%), together with an increase in mononuclear leukocyte counts (by 119%). Infiltrated pleural neutrophil counts in G-CSF-treated rats (G-CSF-r) 5h after the intrapleural injection of zymosanactivated serum were significantly higher (by 155%) than those in control rats (Vehicle-r). In carrageenin-induced pleurisy, counts of infiltrated pleural neutrophils in G-CSF-r 5 and 7h after carrageenin were significantly higher (by 119% and 116%) than those in Vehicle-r. G-CSF treatment increased the volume of pleural exudate and the plasma exudation rate by 122% and 226%, compared to values in Vehicle-r 5h after carrageenin. Cobra venom factor (75 μg/kg, i.v.) significantly reduced pleural neutrophil migration in G-CSF-r (by 53%) and Vehicle-r (by 49%). Bromelain (10 mg/kg, i.v.) and aspirin (100 mg/kg, p.o.) reduced pleural neutrophil migration and reduced exudate volume and plasma exudation. Intrapleural bradykinin-(1–5) and prostaglandin E2 levels were significantly higher in G-CSF-r than in Vehicle-r. The increased neutrophil migration in G-CSF-r may be atributed to enhanced activation of the complement system facilitated by increased plasma exudation due to bradykinin and prostaglandins.

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Authors and Affiliations

  1. Department of Pharmacology, Kitasato University School of Medicine, 228, Sagamihara, Kanagawa, Japan

    M. Ogino, M. Majima, M. Kawamura, K. Hatanaka, M. Saito, Y. Harada & M. Katori

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  1. M. Ogino
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  2. M. Majima
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  3. M. Kawamura
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  4. K. Hatanaka
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  5. M. Saito
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  6. Y. Harada
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  7. M. Katori
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accepted by M. J. Parnham

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Ogino, M., Majima, M., Kawamura, M. et al. Increased migration of neutrophils to granulocyte-colony stimulating factor in rat carrageenin-induced pleurisy: Roles of complement, bradykinin, and inducible cyclooxygenase-2. Inflamm Res 45, 335–346 (1996). https://doi.org/10.1007/BF02252946

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  • DOI: https://doi.org/10.1007/BF02252946

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