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Interleukin-8 (IL-8) is a major neutrophil chemotaxin from human alveolar macrophages stimulated with staphylococcal enterotoxin A (SEA)

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Abstract

Since Staphylococcus aureus is an important human pathogen, and infection of the lungs in characterized by neutrophil infiltration we studied the role of a staphylococcal toxin, enterotoxin A (SEA) on the synthesis and secretion of IL-8 by human alveolar macrophages. As SEA concentration was increased, the IL-8 accumulation in the macrophage conditioned medium increased. The concentration of mRNA encoding IL-8 was also elevated in the macrophage in response to increases in SEA concentration. Although the monocytic cell line U937 was able to respond to SEA and secrete IL-8, treatment with PMA prior to SEA stimulation increased the IL-8 accumulation around fifty fold indicating that maturation of the undifferentiated cell to a more macrophage-like cell facilitated IL-8 accumulation. Stimulating human alveolar macrophages with high concentrations of SEA caused an increase in IL-1 accumulation. However, when the cells were incubated with SEA in the presence of IL-1 receptor antagonist, there was no decrease in IL-8 accumulation. Addition of a neutralizing anti-IL-8 monoclonal antibody to the culture medium of SEA-stimulated macrophages significantly reduced the neutrophil chemotactic activity of the medium. These studies showed that IL-8 is a major neutrophil chemotaxin from human alveolar macrophages stimulated with SEA.

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Authors and Affiliations

  1. Department of Biochemistry, University of Texas Health Center, 75710-2003, Tyler, TX, USA

    E. J. Miller, F. K. Carr, J. M. Noble & A. B. Cohen

  2. Department of Medicine, University of Texas Health Center, 75710-2003, Tyler, TX, USA

    A. B. Cohen

  3. Department of Pharmacology, Kagoshima University Dental School, Kagoshima, Japan

    S. Nagao

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  1. E. J. Miller
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  2. S. Nagao
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  3. F. K. Carr
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  4. J. M. Noble
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  5. A. B. Cohen
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accepted by I. Ahnfelt-Rønne

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Miller, E.J., Nagao, S., Carr, F.K. et al. Interleukin-8 (IL-8) is a major neutrophil chemotaxin from human alveolar macrophages stimulated with staphylococcal enterotoxin A (SEA). Inflamm Res 45, 386–392 (1996). https://doi.org/10.1007/BF02252933

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  • DOI: https://doi.org/10.1007/BF02252933

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