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Dissociation of urease in acetate buffer of pH 3.5 with retention of the enzymatic activity

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  • Chimica. Biochimica. Biophysica
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Riassunto

Le molecole dell'urease (peso molecolare 480,000, coefficiente di sedimentazione 18,6 S) si dividono a metà quando trattate con tampone acetato, 0,1M, pH 3,5. Inizialmente, l'attività enzimatica è 40% di quella al pH 7 (tampone fosfato, 0,34M) e la dissociazione è riversibile. Prolungata esposizione al pH 3,5 causa una lenta denaturazione, con perdita dell'attività e dell'abilità di ricostituirsi.

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References

  1. J. B. Sumner, N. Gralén andI. B. Eriksson-Quensel, J. biol. Chem.125, 37 (1938).

    Article  CAS  Google Scholar 

  2. F. J. Reithel andJ. E. Robbins, Archs Biochem. Biophys.120, 158 (1967).

    Article  CAS  Google Scholar 

  3. G. Gorin andC. C. Chin, Anal. Biochem.17, 49 (1966).

    Article  CAS  PubMed  Google Scholar 

  4. G. Gorin andC. C. Chin, Fedn Proc. Fedn Am. Socs exp. Biol.26, 605 (1967).

    Google Scholar 

  5. G. Mamiya andG. Gorin, Biochim. biophys. Acta105, 382 (1965).

    Article  CAS  PubMed  Google Scholar 

  6. C. C. Chin andG. Gorin, Anal. Biochem.17, 60 (1966).

    Article  CAS  PubMed  Google Scholar 

  7. F. J. Reithel, J. E. Robbins andG. Gorin, Archs Biochem. Biophys.108, 409 (1964).

    Article  CAS  Google Scholar 

  8. G. Gorin, G. Mamiya andC. C. Chin, Experientia23, 443 (1967).

    Article  CAS  PubMed  Google Scholar 

  9. J. M. Creeth andL. W. Nichol, Biochem. J.77, 230 (1960).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. P. P. Seghal, R. J. Tanis andA. W. Naylor, Biochem. biophys. Res. Commun.20, 550 (1965).

    Article  CAS  PubMed  Google Scholar 

  11. K. K. Stewart andL. C. Craig, Fedn Proc. Fedn. Am. Socs exp. Biol.25, 590 (1966).

    Google Scholar 

  12. D. P. Blattler, C. C. Contaxis andF. J. Reithel, Nature216, 275 (1967).

    Article  Google Scholar 

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Authors and Affiliations

  1. Chemistry Department, Oklahoma State University, 74074, Stillwater, Oklahoma, USA

    G. Gorin, C. C. Chin & S. F. Wang

Authors
  1. G. Gorin
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  2. C. C. Chin
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  3. S. F. Wang
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Additional information

This work was supported by grant No. GM 13,900 and by Career Development Award No. 5K3 GM 13,489 (to G. G.) from the National Institutes of Health.

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Gorin, G., Chin, C.C. & Wang, S.F. Dissociation of urease in acetate buffer of pH 3.5 with retention of the enzymatic activity. Experientia 24, 685–687 (1968). https://doi.org/10.1007/BF02138313

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  • DOI: https://doi.org/10.1007/BF02138313

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