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The effect of 2 fixatives, (1) s-Collidin buffered OsO4, (2) phosphate buffered OsO4, on the liver cell was studied by morphometric analysis. After fixation with solution 2, large areas of smooth endoplasmic reticulum were covered by electron-opaque deposits of glycogen. No such masking was noticed by using solution 1. Other cell organelles showed no significant differences in their relative volumes. By using fixative solution 2, decrease in glycogen can simulate relative volume increase of the smooth endoplasmic reticulum.
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Wesentliche Teile der vorliegenden Arbeit wurden von Frl. F.Stockmann der Medizinischen Fakultät der Universität Basel als Inaugural-Dissertation vorgelegt.
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Stockmann, F., Bianchi, L., Rohr, H.P. et al. Morphometrische Untersuchungen an der Rattenleber — Parenchymzelle nach Anwendung verschiedener Fixationspuffer. Experientia 26, 174–176 (1970). https://doi.org/10.1007/BF01895564
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DOI: https://doi.org/10.1007/BF01895564