Skip to main content
SpringerLink
Log in

The oxygen-linked hydrogen ion binding (the Haldane coefficient) of bovine hemoglobin

  • Physiologica
  • Published:
Experientia Aims and scope Submit manuscript

Summary

The Haldane coefficient (the amount of the oxygen-linked hydrogen ion binding of hemoglobin) was determined in bovine erythrolysate (Hb concentration=13.5 mM) by means of the differential titration method with varying PCO2 from 0 to 74 mm Hg and pH from 6.0 to 8.5 at 37°C. The maximum value of the coefficient was found to be 0.49 mM per mM Hb at PCO2=0 and pH 7.20. With increasing of PCO2, the coefficient became smaller in all ranges of pH studied. The coefficient under the conditions of pH 7.20 and PCO2=45 mm Hg that are normally prevailing in the interior of bovine erythrocytes was 0.31.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. O. Siggaard-Andersen, Scand. J. clin Lab. Invest.27, 351 (1971).

    Article  CAS  PubMed  Google Scholar 

  2. O. Siggaard-Andersen,The Acid-Base Status of the Blood, 4th edn. (Munksgaard, Copenhagen 1974), p. 79.

    Google Scholar 

  3. N. Takano, E. Hayashi andK. Matsue, Clin. Physiol.5, 357 (1975), in Japanese.

    Google Scholar 

  4. J. D. Torrance, inErythrocytes, Thrombocytes, Leukocytes. (Ed.E. Gerlach; Georg Thieme Publ., Stuttgart 1973), p. 161.

    Google Scholar 

  5. L. H. van Kempen, P. M. Breepoel andF. Kreuzer, Respir. Physiol.23, 223 (1975).

    Article  PubMed  Google Scholar 

  6. H. F. Bunn, Science.172, 1049 (1971).

    Article  CAS  PubMed  Google Scholar 

  7. S. H. de Bruin, L. H. M. Janssen andG. A. J. van Os, Biochim. biophys. Acta188, 207 (1969).

    Article  PubMed  Google Scholar 

  8. K. A. Evelyn andH. T. Malloy, J. biol. Chem.126, 655 (1938).

    Article  CAS  Google Scholar 

  9. N. Takano, E. Hayashi andK. Matsue, Pflügers Archiv, in press.

  10. J. V. Kilmartin andL. Rossi-Bernardi, Physiol. Rev.53, 836 (1973).

    Article  CAS  PubMed  Google Scholar 

  11. L. Garby, M. Robert andB. Zaar, Acta physiol. scand.84, 482 (1972).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiology, School of Medicine, Kanazawa University, 920, Kanazawa, Japan

    N. Takano & K. Nishikura

Authors
  1. N. Takano
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. Nishikura
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Acknowledgments. We are much obliged to Dr.J. N. Hunt for his valuable discussion during preparation of the manuscript.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Takano, N., Nishikura, K. The oxygen-linked hydrogen ion binding (the Haldane coefficient) of bovine hemoglobin. Experientia 32, 1437–1439 (1976). https://doi.org/10.1007/BF01937419

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01937419

Keywords

Search

Navigation