Skip to main content
SpringerLink
Log in

Nuclease for DNA apurinic sites in chicken liver

  • Specialia
  • Published:
Experientia Aims and scope Submit manuscript

Summary

Chicken liver crude extract produced acid-soluble diphenylamine-positive material in the presence of depurinated and alkylated DNA, while the formation of such material from normal and single stranded DNA was comparatively low. The maximum acid-soluble material produced was not increased further by alkali, indicating that the enzymatic action is mostly directed towards apurinic sites.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. S. M. Hadi and D. A. Goldthwait, Biochemistry10, 4986 (1971).

    Article  CAS  PubMed  Google Scholar 

  2. T. Lindahl and B. Nyberg, Biochemistry11, 3618 (1972).

    Article  CAS  PubMed  Google Scholar 

  3. W. G. Verly and Y. Paquette, Can. J. Biochem.50, 217 (1972).

    Article  CAS  PubMed  Google Scholar 

  4. W. G. Verly and Y. Paquette, Can. J. Biochem.51, 1003 (1973).

    Article  CAS  PubMed  Google Scholar 

  5. A. Andersson and T. Lindahl, J. biol. Chem.249, 1530 (1974).

    Article  Google Scholar 

  6. A. A. Wani and S. M. Hadi, Ind. J. Biochem. Biophys., in press (1976).

  7. W. G. Verly and Y. Paquette, Nature, Lond.244, 67 (1973).

    CAS  Google Scholar 

  8. H. R. Mahler and E. H. Cordes, in: Biological Chemistry, 2nd ed. p. 448. Harper and Row Publishers, N. Y. 1971.

    Google Scholar 

  9. E. Ohtsuka, Y. Tanigawa and S. S. Koide, Experientia31, 175 (1975).

    Article  CAS  PubMed  Google Scholar 

  10. W. C. Schneider, in: Methods in Enzymology, vol. 3, p. 680. Ed. S. P. Colowick and Nathan O. Kaplan. Academic Press, N. Y. 1957.

    Chapter  Google Scholar 

  11. B. Strauss and T. Hill, Biochim. biophys. Acta213, 14 (1970).

    Article  CAS  PubMed  Google Scholar 

  12. C. Tamn, H. S. Shapiro, R. Lipshitz and E. Chargaff, J. biol. Chem.203, 673 (1953).

    Article  Google Scholar 

  13. B. S. Strauss and M. Robbins, Biochim. biophys. Acta161, 68 (1968).

    Article  CAS  PubMed  Google Scholar 

  14. E. C. Friedberg, S. M. Hadi and D. A. Goldthwait, J. biol. Chem.244, 5879 (1969).

    Article  CAS  PubMed  Google Scholar 

  15. W. G. Verly, F. Cossard and P. Crine, Proc. Nat. Acad. Sci. USA71, 2273 (1974).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. D. M. Kirtikar and D. A. Goldthwait, Proc. Nat. Acad. Sci. USA71, 2022 (1974).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biochemistry Division, Department of Chemistry, Aligarh Muslim University, 202001, Aligarh, India

    Rana Y. Rizvi & S. M. Hadi

Authors
  1. Rana Y. Rizvi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. M. Hadi
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Acknowledgment. The authors are grateful to Council of Scientific and Industrial Research, India, for financial assistance to one of us (R. Y. R.).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rizvi, R.Y., Hadi, S.M. Nuclease for DNA apurinic sites in chicken liver. Experientia 33, 418–420 (1977). https://doi.org/10.1007/BF01922186

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01922186

Keywords

Search

Navigation