Skip to main content
Log in

Isolation procedure for bovine retinal rod outer segments

  • Published:
Experientia Aims and scope Submit manuscript

Summary

An isolation procedure to obtain rod outer segments from cattle retinas is reported. Centrifugation of homogenates in discontinuous and continuous sucrose density gradients yields purified photoreceptor cell outer segments. Assay of the final preparation for rhodopsin content gives a ratio of 2.4 for DO280 nm/DO498 nm.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. C. Nicotra, M. A. Livrea, A. Bongiorno and A. Bono, IRCS Med. Sci.3, 530 (1975).

    CAS  Google Scholar 

  2. C. Nicotra and M. A. Livrea, Expl Eye Res.22, 367 (1976).

    Article  CAS  Google Scholar 

  3. C. Nicotra and M. A. Livrea, Ital. J. Biochem.27, 2 (1978).

    Google Scholar 

  4. F. J. M. Daemen, Biochim. biophys. Acta300, 255 (1973).

    Article  CAS  PubMed  Google Scholar 

  5. D. G. McConnell, J. Cell Biol.27, 459 (1965).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. D. G. McConnell, G. W. Ozga and D. A. Solze, Biochim. biophys. Acta184, 11 (1969).

    Article  CAS  PubMed  Google Scholar 

  7. W. J. de Grip, F. J. M. Daemen and S. L. Bonting, Vision Res.12, 1697 (1972).

    Article  PubMed  Google Scholar 

  8. D. Bownds, A. Brodie, W. E. Robinson, D. Palmer, J. Miller and A. Shedlovsky, Expl Eye Res.18, 253 (1974).

    Article  CAS  Google Scholar 

  9. F. Siebert, H. Schmid and R. H. Mull, Biochem. biophys. Res. Commun.75, 1071 (1977).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Livrea, M.A., Nicotra, C., Bongiorno, A. et al. Isolation procedure for bovine retinal rod outer segments. Experientia 36, 894–895 (1980). https://doi.org/10.1007/BF01953777

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01953777

Keywords

Navigation