Summary
Perfusion of the isolated rat heart at constant heart rate and coronary flow with the inhibitor of cyclic nucleotide phosphodiesterase, pentoxifylline (10−4 moles/l), produced no significant effect on the maximum rate and the peak of contraction, but increased the maximum rate of relaxation. cAMP level and cAMP-dependent protein kinase activity were increased in the absence of changes in cGMP. The results were identical in hearts of reserpinized rats.
Similar content being viewed by others
References
R. W. Tsien, in: Advances in Cyclic Nucleotide Research, vol. 8, p. 363. Raven Press, New York 1977.
S. L. Keely and J. D. Corbin, Am. J. Physiol.2, 269 (1977).
M. M. Appleman, W. J. Thompson and T. R.ssell in: Advances in Cyclic Nucleotide Research, vol. 3, p. 65. Raven Press, New York 1973.
W. R. Kukovetz and G. Pöch, in: Advances in Cyclic Nucleotide Research, vol. 1, p. 262. Raven Press New York 1972.
J. E. Kuo, M. Shoji, N. L. Brackett and D. M. Helfmann, J. cyclic Nucleot. Res.4, 463 (1978).
P. V. Sulakhe, N. L. Leung and P. J. St. Louis, Can. J. Biochem.54, 438 (1976).
J. T. Stull and J. E. Buss, J. biol. Chem.252, 851 (1977).
M. Tada, M. A. Kirchberger and A. M. Katz, J. biol. Chem.250, 2640 (1975).
M. L. Entman, G. S. Levey and S. Epstein, Circulation Res.25, 429 (1968).
M. Morad and E. L. Rolett, J. Physiol. London224, 537 (1972).
A. O. Grassi de Gende, A. D. Pérez Alzueta and H. E. Cingolani, Am. J. Physiol.233, 404 (1977).
H. Nawrath, Nature262, 509 (1976).
W. J. George, R. D. Wilkerson and P. J. Kadowitz, J. Pharmac. exp. Ther.184, 228 (1973).
T. W. Rall and T. C. West, J. Pharmac. exp. Ther.139, 269 (1963).
E. W. Sutherland, G. A. Robinson and R. W. Butcher, Circulation37, 279 (1968).
J. R. Blinks, C. B. Olson, B. R. Jewell and P. Bravený, Circulation Res.30, 367 (1972).
P. B. Henry, J. G. Dobson and B. E. Sobel, Circulation Res.36, 392 (1975).
E. G. Krause and A. Wollenberger, in: Cyclic Nucleotides: Mechanisms of Action, chapter 13, p. 229. Wiley, New York 1976.
J. Häggendal, Acta physiol. scand.59, 242 (1963).
W. B. Wastila, J. T. Stull, S. E. Mayer and D. A. Walsh, J. biol. Chem.246, 1996 (1971).
J. D. Corbin and E. M. Reimann, in: Methods in Enzymology, vol. 38, p. 287. Academic Press, New York 1974.
A. L. Steiner, Ch. W. Parker and D. M. Kipnis, J. biol. Chem.247, 1106 (1972).
G. Brooker, J. cyclic Nucleot. Res.3, 407 (1977).
J. Diamond, R. E. Ten Eick and A. J. Trapani, Biochem. biophys. Res. Commun.79, 912 (1977).
A. L. Steiner, A. S. Pagliara, L. R. Caase and D. M. Kipnis, J. biol. Chem.247, 1114 (1972).
C. J. Wiggers and L. N. Katz, Am. J. Physiol.58, 439 (1922).
W. W. Parmley and E. H. Sonnenblick, Am. J. Physiol.216, 1084 (1969).
E. Shinebourne and R. White, Cardiovasc. Res.4, 194 (1970).
Author information
Authors and Affiliations
Additional information
This work was supported by grants from the Consejo Nacional de Investigaciones Cientificas y Técnicas and the Comisión de Investigaciones Científicas. Buenos Aires, Argentina.
We thank Hoechst Laboratories for the partial support and Mrs Alicia R. Ramirez and María Prinzo for the excellent technical assistance.
Rights and permissions
About this article
Cite this article
Vittone, L., Chiappe, L.E., Argel, M.I. et al. The mechanical and biochemical effects of pentoxifylline on the perfused rat heart. Experientia 36, 1088–1090 (1980). https://doi.org/10.1007/BF01965986
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01965986