Summary
Cell-free extracts ofTrypanosoma cruzi contain proteolytic activity able to degrade endogenous substrates. The activity was maximal at pH 3 to 4, had an ‘optimal’ temperature of 65°C, and was strongly inhibited by N-α-p-tosyl-L-lysine chloromethyl ketone.
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This investigation received financial support from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases, the Fundación Julio Cherny, and the Ministerio de Salud Pública y Medio Ambiente de la República Argentina. The authors gratefully acknowledge the technical assistance of Mr Esteban Bontempi.
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Cazzulo, J.J., Franke de Cazzulo, B.M. Proteolytic activity on endogeneous substrates in cell-free extracts ofTrypanosoma cruzi. Experientia 38, 1335–1337 (1982). https://doi.org/10.1007/BF01954936
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DOI: https://doi.org/10.1007/BF01954936