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Rana esculenta complex: An experimental analysis of lethality and hybridogenesis

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Summary

Experiments designed to analyze the lethality and hybridogenesis in the European green frog complex have yielded the following results: 1. As a rule the inter-se cross ofRana esculenta is lethal, but several crosses have produced fully viable progeny. The frequency of such ‘break-through’ crosses appears to be related to parental population structure. 2. Parabiotic joining of lethal to viable embryos indicates that manifestation of the lethal effect is autonomous. There is, however, a 16–18% increase in the life span of the lethal partner. 3. Studies of LDH isozyme patterns revealed that thelessonae-specific alleles coding for the Ba and Bc subunits can be passed to the F1 progeny from a parental female or male of theesculenta phenotype. This demonstrates that there is no total elimination of thelessonae genome in theesculenta germ cells. 4. Immunologically, offspring from the inter-se cross ofR. esculenta show a closer relationship to theridibunda than to theesculenta phenotype. Variations of antigenic protein patterns suggest the possibility of chromosomal recombination betweenlessonae andridibunda in theesculenta hybrid. These results are confirmed by two-dimensional electrophoretic analysis of proteins in the oocytes of the three frog phenotypes.

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Authors and Affiliations

  1. Institute of Zoology, University of Zürich, CH-8057, Zürich, Switzerland

    J. Binkert, P. Borner & P. S. Chen

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  1. J. Binkert
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  2. P. Borner
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  3. P. S. Chen
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Additional information

This work was supported by grants from the Swiss National Science Foundation and the Georges and Antoine Claraz Schenkung.

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Binkert, J., Borner, P. & Chen, P.S. Rana esculenta complex: An experimental analysis of lethality and hybridogenesis. Experientia 38, 1283–1292 (1982). https://doi.org/10.1007/BF01954915

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