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Enzyme immunoassay for arginine vasopressin

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  • Chimica, Biochimica
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Summary

A highly sensitive enzyme immunoassay for arginine vasopressin (AVP) was described. The enzyme used was β-D-galactosidase fromEscherichia coli, and it was coupled to AVP by using the N-hydroxysuccinimide ester of N-(4-carboxycyclohexylmethyl)-maleimide. The complex was then utilized for the enzyme immunoassay, which could detect 0.5 fmoles (or 0.5 pg) of AVP in the assay tube.

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Authors and Affiliations

  1. Department of Anesthesiology and Biochemistry, Medical College of Miyazaki, 5200 Kihara, Kiyotake-cho, Miyazaki-gun, 889-16, Miyazaki, Japan

    T. Uno, K. Uehara, K. Motomatsu, E. Ishikawa & K. Kato

  2. Department of Biochemistry, Institute for Developmental Research, Aichi Prefectural Colony, 480-03, Kasugai, Aichi, Japan

    T. Uno, K. Uehara, K. Motomatsu, E. Ishikawa & K. Kato

Authors
  1. T. Uno
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  2. K. Uehara
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  3. K. Motomatsu
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  4. E. Ishikawa
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  5. K. Kato
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Uno, T., Uehara, K., Motomatsu, K. et al. Enzyme immunoassay for arginine vasopressin. Experientia 38, 786–787 (1982). https://doi.org/10.1007/BF01972270

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  • DOI: https://doi.org/10.1007/BF01972270

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