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In vitro inactivation of the neurotoxic action of β-bungarotoxin by the marine natural product, manoalide

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Summary

The irreversible neurotoxic action of β-bungarotoxin (β-BuTx) can be prevented by preincubation of the toxin with manoalide, a non-steroidal anti-inflammatory agent. Manoalide was also found to inactivate purified phospholipase A2 and thus prevent hydrolysis of phosphatidylcholine. PLA2 is a component found in several neurotoxic venoms and is also a rate limiting enzyme important in phospholipid metabolism and prostaglandin synthesis in man.

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Authors and Affiliations

  1. Marine Science Institute and Department of Biological Sciences, University of California, 93106, Santa Barbara, California, USA

    J. C. de Freitas, L. A. Blankemeier & R. S. Jacobs

  2. Department of Biological Sciences, University of Califonia, 93106, Santa Barbara, CA, USA

    R. S. Jacobs

Authors
  1. J. C. de Freitas
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  2. L. A. Blankemeier
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  3. R. S. Jacobs
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Additional information

This work is a result of research sponsored in part by NOAA-Department of Commerce under Sea Grant No. NA80AA-D-00120 and the California State Resources Agency R/MP-21. The US Government is authorized to produce and distribute reprints for governmental purposes. A preliminary report of aspects of this work appeared in Fedn Proc.42 (1983) 374. We also acknowledge the technical assistance of Dan Rohrer. Dr George Taborski's advice and assistance during the course of this work is gratefully acknowledged.

J. C. de Freitas is a post-doctoral fellow supported by Fundacao de Amparo à Pesquisa do Estado de São Paulo (81/0263-9) and Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (201.171/82), Brazil.

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de Freitas, J.C., Blankemeier, L.A. & Jacobs, R.S. In vitro inactivation of the neurotoxic action of β-bungarotoxin by the marine natural product, manoalide. Experientia 40, 864–865 (1984). https://doi.org/10.1007/BF01951998

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  • DOI: https://doi.org/10.1007/BF01951998

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