Summary
Arylsulfatase activities in biological materials are too low to be detected by the methods available hitherto. A sensitive and specific assay method for arylsulfatase A (AS-A) has been developed in the present study. Ascorbate-2-sulfate is known to be a specific natural substrate of AS-A; the ascorbic acid liberated by the action of AS-A was quantitatively determined using HPLC equipped with an amperometric detector. The method was used to analyze the activity of AS-A in biological materials.
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Inoue, H., Seyama, Y. & Yamashita, S. Specific determination of arylsulfatase A activity. Experientia 42, 33–35 (1986). https://doi.org/10.1007/BF01975881
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DOI: https://doi.org/10.1007/BF01975881