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Occurrence of thiaminase II inSaccharomyces cerevisiae

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Summary

It was found that cell-free extracts ofSaccharomyces cerevisiae contain thiaminase II which hydrolyzes thiamine and thiamine analogs. The possible involvement of this enzyme and thiamine-synthesizing enzymes in thiamine production from thiamine antagonists is discussed.

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Authors and Affiliations

  1. Department of Biochemistry, Kyoto Prefectural University of Medicine, Kamigyo-ku, 602, Kyoto, Japan

    Y. Kimura & A. Iwashima

Authors
  1. Y. Kimura
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  2. A. Iwashima
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Additional information

30 September 1986

Acknowledgments. We thank the late Dr S. Yurugi, Takeda Pharmaceutical Industries, Osaka, for his generous gifts of dimethialium, 2-northiamine, α-hydroxyethylthiamine, hydroxymethylpyrimidine, 2-norhydroxymethylpyrimidine and hydroxyethylthiazole. We also thank Prof. H. Nakayama, Yamaguchi Women's College, for his kind supply ofEscherichia coli 70–17 and 26–43.

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Kimura, Y., Iwashima, A. Occurrence of thiaminase II inSaccharomyces cerevisiae. Experientia 43, 888–890 (1987). https://doi.org/10.1007/BF01951652

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  • DOI: https://doi.org/10.1007/BF01951652

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