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The genetic engineering of production traits in domestic animals

  • Multi-author Review
  • Transgenic vertebrates
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Abstract

The transfer of recombinant DNA by microinjection of embryo pronuclei provides a novel approach to the manipulation of production traits in domestic animals. In this review, several of the key areas currently under investigation are examined and their progress evaluated. These include the manipulation of the endocrine system by altered growth hormone genes and the modification of animal biochemistry by the introduction of the cysteine biosynthetic pathway and the glyoxylate cycle. The possibilities inherent in the alteration of structural proteins important to domestic animal productivity, and some ethical issues relevant to the release of modified animals are also considered. The experimental information obtained so far in the area indicates that transcriptional regulation of the genes and a thorough understanding of the physiological processes involved are both important factors in the practical application of the technique to the improvement of animal productivity.

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References

  1. Armstrong, D. G., Blaxter, K. L., and Graham, N., McC., Utilization of the end products of ruminant digestion. Proc. Br. Soc. Anim. Prod. (1957) 3–15.

  2. Byrne, C. R., The isolation of bacterial acetate metabolism genes and their expression in a eukaryote. Ph.D. thesis, Macquarie University, Sydney 1990.

    Google Scholar 

  3. Byrne, C. R., Mouroe, R. S., Ward, K. A., and Kredich, N. M., DNA sequences of thecysK regions ofS. typhimurium andE. coli and linkage of thecysK regions toptsH. J. Bact.170 (1988) 3150–3157.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Byrne, C. R., Stokes, H. W., and Ward, K. A., Nucleotide sequence of the aceB gene encoding malate synthase A inEscherichia coli. Nucl. Acids Res.16 (1988) 9342.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Cameron, F. H., and Jennings, P. A., Specific gene suppression by engineered ribozymes in monkey cells. Proc. natl Acad. Sci. USA86 (1989) 9139–9143.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Chapman, R. E., and Ward, K. A., Histological and biochemical features of the wool fibre and follicle, in: Physiological and Environmental Limitations to Wool Growth, pp. 193–208. Eds J. L. Black and P. J. Reis. University of New England Publishing Unit, Armidale 1979.

    Google Scholar 

  7. Cioni, M., Pinzauti, G., and Vanni, P., Comparative biochemistry of the glyoxylate cycle. Comp. Biochem. Physiol.70B (1981) 1–26.

    CAS  Google Scholar 

  8. Clark, A. J., Bessos, H., Bishop, J. O., Brown, P., Harris, S., Lathe, R., McClenaghan, M., Prowse, C., Simons, J. P., Whitelaw, C. B. A., and Wilmut, I., Expression of human anti-hemophilic factor IX in the milk of transgenic sheep. Biotechnology7 (1989) 487–492.

    CAS  Google Scholar 

  9. Costantini, F., and Lacy, E., Introduction of a rabbit beta-globin gene into the mouse germ line. Nature294 (1981) 92–94.

    Article  CAS  PubMed  Google Scholar 

  10. Cotten, M., and Birnstiel, M. L., Ribozyme mediated destruction of RNAin vivo. Embo J.8 (1989) 3861–3866.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. D'Andrea, R. J., Sivaprasad, A. V., Bawden, C. S., Kuczek, E. S., Whitbread, L. A., and Rogers, G. E., Isolation of microbial genes for cysteine synthesis and prospects for their use in increasing wool growth, in: The Biology of Wool and Hair, pp. 447–464. Eds G. E. Rogers, P. J. Reis, K. A. Ward and R. G. Marshall. Chapman and Hall, New York and London 1989.

    Google Scholar 

  12. Denk, D., and Bock, A., L-cysteine biosynthesis inEscherichia coli: nucleotide sequence and expression of the serine acetyltransferase (cysE) gene from the wild-type and a cysteine-excreting mutant. J. gen. Microbiol.133 (1987) 515–525.

    CAS  PubMed  Google Scholar 

  13. Ferguson, K. A., The protection of dietary proteins and amino acids against microbial fermentation in the rumen, in: Digestion and Metabolism in the Ruminant, pp. 448–464. Eds I. W. McDonald and A. C. I. Warner. University of New England Publishing Unit, Armidale 1975.

    Google Scholar 

  14. Fraser, R. D. B., MacRae, T. P., and Rogers, G. E., Keratins, Their Composition, Structure and Biosynthesis. Charles C. Thomas, Springfield 1972.

    Google Scholar 

  15. Frenkel, M. J., Powell, B. C., Ward, K. A., Sleigh, M. J., and Rogers, G. E., The keratin BIIIB family: isolation of cDNA clones and structure of a gene and a related pseudogene. Genomics4 (1989) 182–191.

    Article  CAS  PubMed  Google Scholar 

  16. Gordon, J. W., Integration and stable germ line transmission of genes injected into mouse pronuclei. Science214 (1981) 1244–1246.

    Article  CAS  PubMed  Google Scholar 

  17. Hammer, R. E., Pursel, V. G., Rexroad, C. E., Wall, R. J., Bolt, D. J., Palmiter, R. D., and Brinster, R. L., Genetic engineering of mammalian embryos. J. Anim. Sci.63 (1986) 269–278.

    Article  CAS  PubMed  Google Scholar 

  18. Hammer, R. E., Pursel, V. G., Rexroad, C. E. Jr, Wall, R. J., Bolt, D. J., Ebert, K. M., Palmiter, R. D., and Brinster, R. L., Production of transgenic rabbits, sheep and pigs by microinjection. Nature315 (1985) 680–683.

    Article  CAS  PubMed  Google Scholar 

  19. Lindsay, D. B., Metabolism in the whole animal. Proc. Nutr. Soc.38 (1979) 295–301.

    Article  CAS  PubMed  Google Scholar 

  20. Maddocks, I. G., and Jackson, N., Structural Studies of Sheep, Cattle and Goat Skin. CSIRO Division of Animal Production, Blacktown 1988.

    Google Scholar 

  21. Matsuoka, M., and McFadden, B. A., Isolation, hyperexpression and sequencing of theaceA gene encoding isocitrate lyase inEscherichia coli. J. Bact.170 (1988) 4528–4563.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. McKinnon, P. J., Powell, B. C., and Rogers, G. E., Structure and expression of genes for a class of cysteine-rich proteins of the cuticle layers of differentiating wool and hair follicles. J. Cell Biol.111 (1990) 2587–2600.

    Article  Google Scholar 

  23. Murray, J. D., Nancarrow, C. D., Marshall, J. T., Hazelton, I. G., and Ward, K. A., Production of transgenic sheep by microinjection of ovine metallothionein-ovine growth hormone fusion genes. Reprod. Fert. Devl1 (1989) 147–155.

    Article  CAS  Google Scholar 

  24. Nancarrow, C. D., Marshall, J. T. A., Clarkson, J. L., Murray, J. D., Millard, R. M., Shanahan, C. M., Wynn, P. C., and Ward, K. A., Expression and physiology of performance regulating genes in transgenic sheep. J. Reprod. Fert.43, suppl. (1991) 277–291.

    CAS  Google Scholar 

  25. Palmiter, R. D., and Brinster, R. L., Germline transformation of mice. Rev. Genet.20 (1986) 465–500.

    Article  CAS  Google Scholar 

  26. Palmiter, R. D., Brinster, R. L., Hammer, R. E., Trumbauer, M. E., Rosenfeld, M. G., Birnberg, N. C., and Evans, R. M., Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone GH fusion genes stimulate growth in mice. Nature300 (1982) 611–615.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Palmiter, R. D., Norstedt, R. E., Gelinas, R. E., Hammer, R. E., and Brinster, R. L., Metallothionein-human GH fusion genes stimulate growth in mice. Science222 (1983) 809–814.

    Article  CAS  PubMed  Google Scholar 

  28. Powell, B. C., Cam, G. R., Feitz, M. J. and Rogers, G. E., Clustered arrangement of keratin intermediate filament genes. Proc. natl Acad. Sci. USA83 (1986) 5048–5052.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Powell, B. C., and Rogers, G. E., Cyclic hair-loss and regrowth in transgenic mice overexpressing an intermediate filament gene. Embo J.9 (1990) 1485–1493.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Powell, B. C., Sleigh, M. J., Ward, K. A., and Rogers, G. E., Mammalian keratin gene families: organisation of genes coding for the B2 high-sulphur proteins of sheep wool. Nucl. Acids Res.11 (1983) 5327–5346.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Pursel, V. G., Pinkert, C. A., Miller, K. F., Bolt, D. J., Campbell, R. G., Palmiter, R. D., Brinster, R. L., and Hammer, R. E., Genetic engineering of livestock. Science244 (1989) 1281–1288.

    Article  CAS  PubMed  Google Scholar 

  32. Pursel, V. G., Rexroad, C. E. Jr, Bolt, D. J., Miller, K. F., Wall, R. J., Hammer, R. E., Pinkert, C. A., Palmiter, R. D. and Brinster, R. L., Progress on gene transfer in farm animals. Vet. Immunol. Histopath.17 (1987) 303–312.

    Article  CAS  Google Scholar 

  33. Rees, W. D., Flint, H. J., and Fuller, M. F., A molecular biological approach to reducing dietary amino acid needs. Biotechnology8 (1990) 629–633.

    CAS  PubMed  Google Scholar 

  34. Reis, P. J., The growth and composition of wool. Aust. J. biol. Sci.20 (1967) 809–825.

    Article  CAS  PubMed  Google Scholar 

  35. Reis, P. J., and Schinkel, P. G., Some effects of sulphur-containing amino acids on the growth and composition of wool. Aust. J. biol. Sci.16 (1963) 218–230.

    Article  CAS  Google Scholar 

  36. Reis, P. J., Tunks, D. A., and Downes, A. M., The influence of abomasal and intravenous supplements of sulphur-containing amino acids on wool growth rate. Aust. J. biol. Sci.26 (1973) 249–258.

    Article  CAS  PubMed  Google Scholar 

  37. Reis, P. J., Tunks, D. A., and Sharry, L. F., Plasma amino acid patterns in sheep receiving abomasal infusions of methionine and cystine. Aust. J. biol. Sci.26 (1973) 635–644.

    Article  CAS  PubMed  Google Scholar 

  38. Rexroad, C. E. Jr, Behringer, R. R., Bolt, D. J., Miller, K. F., Palmiter, R. D., and Brinster, R. L., Insertion and expression of a growth hormone fusion gene in sheep. J. Anim. Sci.6 (1988) 267.

    Google Scholar 

  39. Rexroad, C. E. Jr, Hammer, R. E., Behringer, R. R., Palmiter, R. D., and Brinster, R. L., Insertion, expression and physiology of growth-regulating geres in ruminants. J. Reprod. Fert.41, suppl. (1990) 119–124.

    CAS  Google Scholar 

  40. Rexroad, C. E. Jr, Hammer, R. E., Bolt, D. J., Mayo, K. M., Frohman, L. A., Palmiter, R. D., and Brinster, R. L., Production of transgenic sheep with growth regulating genes. Molec. Reprod. Devl.1 (1989) 164–169.

    Article  CAS  Google Scholar 

  41. Rexroad, C. E. Jr, and Pursel, V. G., Status of gene transfer in domestic animals. Proc. 11th Int. Congr. Anim. Reprod. and A. I.5 (1988) 29–35.

    Google Scholar 

  42. Rieul, C., Bleicher, F., Duclos, B., Cortay, J. C., and Cozzone, A. J., Nucleotide sequence of the aceA gene coding for isocitrate lyase inEscherichia coli. Nucl. Acids Res.16 (1988) 5689–5689.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Rogers, R. E., Improvement of wool production through genetic engineering. Trends Biotechnol.8 (1990) 6–11.

    Article  CAS  PubMed  Google Scholar 

  44. Roschlau, K., Rommel, P., Andreewa, L., Zackel, M., Roschlau, D., Zackel, B., Schwerin, M., Huhn, R., and Gazarjan, K. G., Gene transfer experiments in cattle. J. Reprod. Fert.38, suppl. (1989) 153–160.

    CAS  Google Scholar 

  45. Roschlau, K., Rommel, P., Roschlau, D., Schwiderski, H., Huhn, R., Kanitz, W., and Rehbock, F., Microinjection of viral vector in bovine zygotes. Arch. Tierz. Berlin31 (1988) 3–8.

    Google Scholar 

  46. Shanahan, C. M., Rigby, N. W., Murray, J. D., Marshall, J., Townrow, C., Nancarrow, C. D., and Ward, K. A., Regulation of expression of a sheep growth hormone fusion gene in transgenic mice. Molec. cell. Biol.9 (1989) 5473–5479.

    CAS  PubMed  PubMed Central  Google Scholar 

  47. Simons, J. P., Wilmut, I., Clark, A. J., Archibald, A. L., Bishop, J. O., and Lathe, R., Gene transfer into sheep. Biotechnology6 (1988) 179–183.

    CAS  Google Scholar 

  48. Sivaprasad, A. V., D'Andrea, R. J., Bawden, C. S., Kuczek, E. S., and Rogers, G. E., Towards a new sheep genotype with increased wool growth by transgenesis with microbial genes for cysteine synthesis. J. cell. Biochem.13B, suppl. (1989) 183.

    Google Scholar 

  49. Stewart, T., Wagner, E. F., and Mintz, B., Human beta-globin gene sequences injected into mouse eggs, retained in adults, and transmitted to progeny. Science217 (1982) 1046–1048.

    Article  CAS  Google Scholar 

  50. Van Soest, P. J., Nutritional Ecology of the Ruminant. Durham and Downey, Portland 1982.

    Google Scholar 

  51. Vize, P. D., Mickalska, A. E., Ashman, R., Lloyd, B., Stone, B. A., Quinn, P., Wells, J. R. E., and Seamark, R. F., Introduction of a porcine growth hormone fusion gene into transgenic pigs promotes growth. J. Cell Sci.90 (1988) 295–300.

    Article  CAS  PubMed  Google Scholar 

  52. Wagner, E. F., Stewart, T. A., and Mintz, B., The human beta-globin gene and a functional viral thymidine kinase gene in developing mice. Proc. natl Acad. Sci. USA78 (1981) 5016–5020.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  53. Wagner, T. E., Development of transgenic pigs. J. Cell Biochem.13B, suppl. (1989) 164.

    Google Scholar 

  54. Ward, K. A., Byrne, C. R., Wilson, B. W., Leish, Z., Rigby, N. W., Townrow, C. R., Hunt, C. L., Murray, J. D., and Nancarrow, C. D., The regulation of wool growth in transgenic animals. Adv. Dermatol.1 (1991) 70–76.

    Google Scholar 

  55. Ward, K. A., Murray, J. D., Nancarrow, C. D., Boland, M. P., and Sutton, R., The role of embryo gene transfer in sheep breeding programmes, in: Reproduction in sheep, pp. 279–285. Eds D. R. Lindsay and D. T. Pearce. Australian Wool Corporation, Melbourne 1984.

    Google Scholar 

  56. Ward, K. A., Murray, J. D., Shanahan, C. M., Rigby, N. W., and Nancarrow, C. D., The creation of transgenic sheep for increased wool productivity, in: The Biology of Wool and Hair, pp. 465–477. Eds G. E. Rogers, P. J. Reis, K. A. Ward and R. C. Marshall. Chapman and Hall, London and New York 1988.

    Chapter  Google Scholar 

  57. Ward, K. A., Nancarrow, C. D., Byrne, C. R., Shanahan, C. M., Murray, J. D., Leish, Z., Townrow, C., Rigby, N. W., Wilson, B. W., and Hunt, C. L., The potential of transgenic animals for improved agricultural productivity. Rev. sci. tech. Off. int. Epiz.9 (1990) 847–864.

    Article  CAS  Google Scholar 

  58. Ward, K. A., Nancarrow, C. D., Murray, J. D., Wynn, P. C., Speck, P., and Hales, J. R. S., The physiological consequences of growth hormone fusion gene expression in transgenic sheep. J. Cell Biochem.13 (1989) 164.

    Google Scholar 

  59. Ward, K. A., Sleigh, M. J., Powell, B. C., and Rogers, G. E., The isolation and analysis of the major wool keratin gene families, in: Proceedings of the 2nd World Congress on Genetics Applied to Livestock production, vol. 6, pp. 146–156, 1982.

  60. Wilson, B. W., Edwards, K. J., Sleigh, M. J., Byrne, C. R., and Ward, K. A., Complete sequence of a type-1 microfibrillar wool keratin gene. Gene73 (1988) 21–31.

    Article  CAS  PubMed  Google Scholar 

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  1. CSIRO Division of Animal Production, P.O. Box 239, 2148, Blacktown, N.S.W., Australia

    K. A. Ward & C. D. Nancarrow

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Ward, K.A., Nancarrow, C.D. The genetic engineering of production traits in domestic animals. Experientia 47, 913–922 (1991). https://doi.org/10.1007/BF01929882

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