Abstract
Glutamate (glu) an excitatory neurotransmitter amino acid, is present in high concentrations in the mammalian central nervous system and is the most abundant amino acid in our daily diet. In the present study the activities of lactate dehydrogenase (LDH) and glutamate dehydrogenase (GDH) were evaluated in the circumventricular organs (CVO) of the brain in 25-day-old rats following MSG administration at a dose of 4 mg/g b.wt during the first ten days of life. The results show the LDH activity increased to 265% of that in the control (p<0.001), whereas GDH activity was significantly decreased (p<0.05), The great elevation in LDH, a cytoplasmic marker enzyme, is apparently due to cytoskeletal changes brought about as a consequence of glu toxicity, whereas lowered GDH activity indicates altered glu homostasis in the blood-brain-barrier deficient areas following neonatal exposure to glu.
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References
Engelsen, B., Acta neurol scand.74 (1986) 337.
Fonnum, F., J. Neurochem.42 (1984) 1.
Watkins, J. C., and Evans, R. H., A. Rev. Pharmac. Toxic.21, (1981) 165.
Filer, L. J. Jr., Gavattini, S., Kare, M. R., Reynolds, W. A., and Wurtman, R. J., (Eds), Glutamic acid: Advances in Biochemistry and Physiology, Raven Press, New York (1979) p. 25.
Coyle, J. T., and Schwarcz, R., Nature263 (1976) 244.
Coyle, J. T., Bird, S. J., Evans, R. H., Gulley, R. L., Nader, J. V., Nicklas, W. J. and Olney, J. W., Neurosci. Res. Prog. Bull.19 (1981) 331.
Olney, J. W., in: Kainic Acid as a Tool in Neurobiology, p. 95. Eds E. G. McGeer, J. W. Olney and P. L. McGeer. Raven Press, New York 1978.
Plaitakis, A., Ann. Neurol.28 (1990) 3.
Bawari, M., Babu, G. N., and Misra, U. K., Annual Meeting of the Indian Academy of Neuroscience, Abstr. P 05. Lucknow 1992.
Weindl, A., in: Frontiers in Neuroendocrinology, p. 1. Eds L. Martini and W. F. Ganong, Oxford University Press, London 1973.
Wroblewski, F., and Ladue, J. S., Proc. Soc. exp. Biol. Med.90 (1955) 210.
Coyle, J. T., J. Neurochem.41 (1983) 1.
Lowry, O. H., Rosebrough N. J., Farr A. I., and Randall, R. J., J. biol. Chem.193 (1951) 265.
Ravindranadh, V., Pai, K. S., and Shankar, S. K., The 4th Indo-US symposium and Workshop on Neuroceptor Signal Transduction Mechanisms, Abstr. P 09. New Delhi, 1991.
Moody, W., A. Rev. Neurosci.7 (1984) 257.
Erecinska, M., and Silver, I. A., Prog. Neurobiol.35 (1990) 245.
Plum, F., Posner, J., and Troy, B., Archs Neurol.18 (1968) 1.
Silver, I. A., and Erecinska, M. J., Genphysiologie95 (1990) 837.
Perez, V. J., and Olney, J. W., J. Neurochem.19 (1972) 1777.
Plaitakis, A., Berl, S., and Yahr, M. D., Science216 (1982) 193.
Plaitakis, A., Adv. Neurol.41 (1984) 225.
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Bawari, M., Babu, G.N., Ali, M.M. et al. Lactate dehydrogenase and glutamate dehydrogenase activities in the circumventricular organs of rat brain following neonatal monosodium glutamate. Experientia 49, 1092–1094 (1993). https://doi.org/10.1007/BF01929919
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DOI: https://doi.org/10.1007/BF01929919