Abstract
The gap junctional intercellular communication (GJIC) determined by measuring dye coupling with Lucifer yellow, decreased within 3 d from 66% to 28% in monocultures of rat liver parenchymal cells. Coculturing of the parenchymal cells with a nonparenchymal epithelial cell line from rat liver resulted in increased and stabilized intercellular communication (83% after 3 d). The presence of isolated plasma membrane vesicles of the nonparenchymal epithelial cells also stabilized the intercellular communication between the liver parenchymal cells (70% after 3 d). When liver parenchymal cells were cocultured with a rat liver fibroblast cell line the gap junctional communication between the parenchymal cells was not stabilized (43% after 3 d), and isolated plasma membrane vesicles of the fibroblast were also unable to support the GJIC in parenchymal cells (35% after 3 d). It is concluded that plasma membrane constituents of the nonparenchymal epithelial cells were responsible for the stabilization of the GJIC between parenchymal cells. A heterotypic gap junctional communication between parenchymal and nonparenchymal cells was not observed.
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Spray, D. C., Ginzberg, R. D., Morales, E. A., Gatmaitan, Z., and Arias, I. M., J. Cell Biol.103 (1986) 135.
Guguen-Guillouzo, C., Clement, B., Baffet, G., Beaumont, C., Morel-Chany, E., Glaise, D., and Guillouzo, A., Expl Cell Res.143 (1983) 47.
Begue, J. M., Guguen-Guillouzo, C., Pasdeloup, N., and Guillouzo, A., Hepatology4 (1984) 839.
Utesch, D., and Oesch, F., In vitro cell. Dev. Biol.28 (1992) 193.
Fausto, N., in: In Mouse Liver Carcinogenesis: Mechanisms and Species Comparisons, p. 325. Ed. D. E. Stevenson. Wiley-Liss, New York 1990.
Noda, C., Ohguri, M., and Ichihara, A., Biochem. biophys. Res. Commun.168 (1990) 335.
Ben-Zeev, A., Robinson, G. S., Bucher, N. L. R., and Farmer, S. R., Proc. natl Acad. Sci. USA85 (1988) 335.
Kumatori, A., Nakamura, T., and Ichihara, A., Biochem. biophys. Res. Commun.178 (1991) 480.
Niimi, S., Hayakawa, T., and Tanaka, A., Biochem. biophys. Res. Commun.174 (1991) 928.
Mesnil, M., Fraslin, J. M., Picolli, C., Yamasaki, H., and Guguen-Guillouzo, C., Expl Cell Res.173 (1987) 524.
Wang, S. R., Renaud, G., Infante, J., Catala, D., and Infante, R., In Vitro cell. Dev. biol.21 (1985) 526.
Scott, R. E., Science194 (1976) 743.
Markwell, M. A. K., Haas, S. Z., Bieber, L. L., and Tolbert, N. E., Analyt. Biochem.87 (1978) 206.
Jefferson, D. M., Clayton, D. F., Darnell, J. E., and Reid, L., Molec. cell. Biol.4 (1984) 1929.
Utesch, D., Molitor, E., Platt, K. L., and Oesch, F., In vitro cell. Dev. Biol.27 (1991) 858.
Nakamura, T., Yoshimoto, K., Nakayama, Y., Tomita, Y., and Ichihara, A., Proc. natl Acad. Sci. USA80 (1983) 7229.
Corlu, A., Kneip, B., Lhadi, C., Leray, G., Glaise, D., Baffet, G., Bourel, D., and Guguen-Guillouzo, C., J. Cell Biol.115 (1991) 505.
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Diener, B., Beer, N., Dürk, H. et al. Gap junctional intercellular communication of cultured rat liver parenchymal cells is stabilized by epithelial cells and their isolated plasma membranes. Experientia 50, 124–126 (1994). https://doi.org/10.1007/BF01984948
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DOI: https://doi.org/10.1007/BF01984948