Summary
The axolemma of nonmyelinated fibres from the corpus callosum and cerebellar cortex (C.N.S.) and the vagus nerve (P.N.S.) was investigated with freeze-fracture electron microscopy. The major observations of this study are as follows: (1) there is a highly asymmetrical distribution of intramembranous particles between the E- and P-fracture faces in both C.N.S. and P.N.S. fibres; (2) the total number of particles on the P-faces of all axonal types studied is considerably greater than that on the E-face; (3) the number of particles on the E-faces of C.N.S. axons is greater than that on the E-faces of P.N.S. axons; and (4) the percentage of large (>9.6 nm) particles is greater on the E-face than on the P-face regardless of the axon studied. The results are compared with previous freeze-fracture investigations on the nodal and internodal membranes of myelinated fibres.
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Black, J. A., Foster, R. E. &Waxman, S. G. (1981) Freeze-fracture ultrastructure of adult and developing optic nerves.Anatomical Record 199, 27a.
Branton, D. S., Bullivant, S., Gilula, N. B., Karnovsky, M. J., Moor, H., Muhlenthaler, K., Northcote, D. H., Packer, L., Satir, B., Satir, P., Speth, V., Staehelin, L. A., Steere, R. L. &Weinstein, R. S. (1975) Freeze-etching nomenclature.Science 190, 54–6.
Devine, C. E., Simpson, F. O. &Bertaud, W. S. (1971) Freeze-etch studies on the innervation of mesenteric arteries and vas deferens.Journal of Cell Science 9, 411–25.
Ellisman, M. H. (1979) Molecular specializations of the axon membrane at nodes of Ranvier are not dependent upon myelination.Journal of Neurocytology 8, 719–35.
Fox, C. A. &Barnard, J. W. (1957) A quantitative study of the Purkinje cell dendritic branchlets and their relationship to afferent fibres.Journal of Anatomy 91, 299–313.
Kristol, C., Akert, K., Sandri, C., Wyss, U. R., Bennett, M. V. L. &Moor, H. (1977) The Ranvier nodes in the neurogenic electric organ of the knifefishSternarchus: A freeze-etching study on the distribution of membrane-associated particles.Brain Research 125, 197–212.
Kristol, C., Sandri, C. &Akert, K. (1978) Intramembranous particles at the nodes of Ranvier of the cat spinal cord: a morphometric study.Brain Research 142, 391–400.
Livingston, R. B., Pfenninger, K., Moor, H. &Akert, K. (1973) Specialized paranodal and interparanodal glial-axonal junctions in the peripheral and central nervous system: a freeze-etching study.Brain Research 58, 1–24.
Palay, S. &Chan-Palay, V. (1974) Granule cells.Cerebellar Cortex: Cytology and Organization, pp. 88–99. New York: Springer-Verlag.
Peracchia, C. (1974) Excitable membrane ultrastructure: I. Freeze-fracture of crayfish axons.Journal of Cell Biology 51, 107–22.
Ritchie, J. M. &Rogart, R. B. (1977) Density of sodium channels in mammalian myelinated fibres and nature of the axonal membrane under the myelin sheath.Proceedings of the National Academy of Sciences (U.S.A.) 74, 211–5.
Ritchie, J. M., Rogart, R. B. &Strichartz, G. (1976) A new method for labelling saxitoxin and its binding to nonmyelinated fibres of the rabbit vagus, lobster walking leg and garfish olfactory nerves.Journal of Physiology 261, 477–94.
Rosenbluth, J. (1976) Intramembranous particle distribution at the node of Ranvier and adjacent axolemma in myelinated axons of the frog brain.Journal of Neurocytology 5, 731–45.
Rosenbluth, J. (1978) Glial specializations in extraparanodal regions.Journal of Neurocytology 7, 709–19.
Sandri, C., Van Buren, J. M. &Akert, K. (1977) Membrane morphology of the vertebrate nervous system. A study with freeze-etch technique.Progress in Brain Research, Vol. 46, Amsterdam: Elsevier.
Schnapp, B. &Mugnaini, E. (1978) Membrane architecture of myelinated fibres as seen by freeze-fracture. InPhysiology and Pathobiology of Axons (edited byWaxman, S. G.), pp. 83–124, New York: Raven Press.
Schnapp, B., Peracchia, C. &Mugnaini, E. (1976) The paranodal axo-glial junction in the central nervous system studied with thin sections and freeze-fracture.Neuroscience 1, 181–90.
Simionescu, N. &Simionescu, M. (1976) Galloyl-glucoses of low molecular weight as mordant in electron microscopy. I. Procedure, and evidence of mordanting effect.Journal of Cell Biology 70, 608–21.
Tao-Cheng, J. H. &Rosenbluth, S. (1980) Nodal and paranodal membrane structure in complementary freeze-fracture replicas of amphibian peripheral nerves.Brain Research 199, 249–65.
Waxman, S. G. &Swadlow, H. A. (1976) Ultrastructure of visual callosal axons in the rabbit.Experimental Neurology 53, 115–27.
Wiley, C. A. &Ellisman, M. H. (1980) Rows of dimeric-particles within the axolemma and juxtaposed particles within glia, incorporated into a new model for the paranodal glial-axonal junction at the node of Ranvier.Journal of Cell Biology 84, 261–80.
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Black, J.A., Foster, R.E. & Waxman, S.G. Freeze-fracture ultrastructure of rat C.N.S. and P.N.S. nonmyelinated axolemma. J Neurocytol 10, 981–993 (1981). https://doi.org/10.1007/BF01258525
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DOI: https://doi.org/10.1007/BF01258525