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Innervation and reinnervation of rat inferior olive by neurons containing serotonin and substance P: An immunohistochemical study after 5,6-dihydroxytryptamine lesioning

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Journal of Neurocytology

Summary

Serotonin (5-HT) and substance P (SP) immunoreactive axon terminals were visualized in the inferior olivary complex (IOC) of adult rats, 1 to 2 weeks or 6 to 12 months after cerebro-ventricular injection of 5,6-dihydroxytryptamine (5,6-DHT). In normal or saline-injected controls of the same age, there was some overlap between the respective distributions of 5-HT- and SP-immunostained axonal varicosities among the various subdivisions of IOC. At short time intervals after the 5-HT axotomy, almost as many degenerating axonal profiles showed immunoreactivity to SP as to 5-HT throughout the IOC, suggesting the coexistence of both transmitters within the same fibres. A few areas continued to exhibit characteristic patches of ‘normal-looking’ SP immunoreactivity, consistent with a distinct innervation by SP fibres without coexistent 5-HT. At prolonged survival times after 5,6-DHT treatment, there was a massive increase in the number — and striking similarity in the distribution — of IOC axonal varicosities immunostained for SP as well as for 5-HT. This neo-innervation involved certain subdivisions of the IOC normally receiving fibres of either type (e.g. dorsal accessory olive), but also others normally poor in 5-HT and/or SP (e.g. medial accessory olive). It remains to be determined if this abundance of 5-HT-SP terminals in the ‘hyperinnervated’ IOC reflected a particular capacity to express both transmitters in regenerating 5-HT neurons.

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Authors and Affiliations

  1. Centre de recherche en sciences neurologiques, Département de physiologie, Faculté de médecine, Université de Montréal, H3C 3J7, Montréal, Québec, Canada

    Michel Paré & Laurent Descarries

  2. Laboratoire de Physiologie Nerveuse, CNRS, 91190, Gif-sur-Yvette, France

    Leif Wiklund

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  1. Michel Paré
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  2. Laurent Descarries
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  3. Leif Wiklund
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Paré, M., Descarries, L. & Wiklund, L. Innervation and reinnervation of rat inferior olive by neurons containing serotonin and substance P: An immunohistochemical study after 5,6-dihydroxytryptamine lesioning. J Neurocytol 16, 155–167 (1987). https://doi.org/10.1007/BF01795300

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