Skip to main content
SpringerLink
Log in

Preparation of extracts of culture liquids for gas-chromatographic determination of acidic fermentation products

  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

An extraction method coupled with gas-chromatographic analysis has been devised for the determination of small amounts of C1-C7 branched and straight-chain saturated fatty acids as well as 18 mono-, di- and tricarboxylic acids and related compounds, the latter being analyzed either as their methylesters or as their trimethylsilyl-derivatives. Methods for extracting non-volatile acids or both volatile and non-volatile acids are described for large and small volumes of culture media. The proposed methods have been successfully applied to the determination of non-acidic fermentation products, known amounts of alcohols, and to volatile and non-volatile acids in bacterial fermentation solutions, namely a batch culture ofLactobacillus casei subsp.rhamnosus ATCC 7469. A carbon balance sheet of this organism's metabolic behaviour throughout growth is proposed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bachur, N. R. 1965. A method for detecting organic acids on thin-layer and paper chromatograms. - Anal. Biochem.13: 463–468.

    Google Scholar 

  • Doelle, H. W. 1966. Preparation of extracts of culture liquids for gas-chromatographic determination of non-acidic fermentation products. - Antonie van Leeuwenhoek32: 373–380.

    Google Scholar 

  • Doelle, H. W. 1969. Gas Chromatographic separation and determination of micro quantities of C1-C7 branched and straight-chain saturated fatty acids. - J. Chromatog.39: 398–406.

    Google Scholar 

  • Doelle, H. W. andManderson, G. J. 1969. A rapid quantitative determination method for lactic acid as fermentation endproduct. - J. Gen. Microbiol.

  • Huggett, A. St. G. andNixon, D. A. 1957. Enzymic determination of blood glucose. - Biochem. J.66: 12P.

  • Harmon, M. A. andDoelle, H. W. 1969. Gas Chromatographic separation and determination for microquantities of the esters of the tricarboxylic acid cycle acids and related compounds. - J. Chromatog.42: 157–169.

    Google Scholar 

  • James, A. T. 1960. Qualitative and quantitative determination of the fatty acids by gas-liquid chromatography. IV. Preparation of stationary phases,In D. Glick, [ed.]. Methods Biochem. Anal. Vol. 8. - Interscience Publ., New York.

    Google Scholar 

  • Neish, A. C. 1952. Analytical methods for bacterial fermentations, 2nd revision. - Nat. Res. Council of Canada Report No. 46-8-3.

  • Report by the Taxonomic Subcommittee on lactob: cilli and closely related organisms (1968). Type strains ofLactobacillus species. - Ameri in Type Culture Collection, Rockville, Maryland, U.S.A.

  • Skerman, V. B. D. 1967. A guide to the identification of the genera of bacteria, 2nd ed. - Williams and Wilkins, Baltimore, p. 247.

    Google Scholar 

  • Ting, I. P. andDugger, W. D., Jr. 1965. Separation and detection of organic acids on silica gel. - Analyt. Biochem.12: 571–578.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, University of Queensland, Medical School, Herston, 4006, Brisbane, Australia

    H. W. Doelle & G. J. Manderson

Authors
  1. H. W. Doelle
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. J. Manderson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Doelle, H.W., Manderson, G.J. Preparation of extracts of culture liquids for gas-chromatographic determination of acidic fermentation products. Antonie van Leeuwenhoek 35, 467–478 (1969). https://doi.org/10.1007/BF02219165

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02219165

Keywords

Search

Navigation