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Intergeneric transformation betweenRhizobium andAzotobacter

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Abstract

Attempts were made to transform two strains ofAzotobacter, viz.A. chroococcum andA. vinelandii with the DNA ofRhizobium leguminosarum, R. japonicum, R. meliloti andR. trifoli. Resistance to streptomycin or crystal violet was used as marker for screening purposes. The frequency of transformation varied between 0.05 to 0.95 percent. The transformants also acquired a few other characters of the donor strains.

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References

  • Allen, E. K. andAllen, O. N. 1950. Biochemical and symbiotic properties of the rhizobia. - Bacteriol. Rev.14: 273–330.

    Google Scholar 

  • Balaasa, G. 1963. Genetic transformation ofRhizobium: A review of the works of R. Balaasa. - Bacteriol. Rev.27: 228.

    Google Scholar 

  • Bergey's Manual of Determinative Bacteriology. 1957, 7th edition. Ed. by R. S. Breed, E. G. D. Murray and N. R. Smith. - Williams & Wilkins Co., Baltimore, U.S.A.

    Google Scholar 

  • DeLey, J. andPark, I. W. 1966. Molecular biological taxonomy of some free-living nitrogen-/fixing bacteria. - Antonie van Leeuwenhoek32: 6–16.

    Google Scholar 

  • DeLey.J. andRassel, A. 1965. DNA base composition, flagellation and taxonomy of the genusRhizobium. - J. Gen. Microbiol.41: 85–91.

    Google Scholar 

  • Kleczkowska, J. 1965. Mutations in symbiotic effectiveness inRhizobium trifolii caused by transforming DNA and other agents. - J. Gen. Microbiol.40: 377–383.

    Google Scholar 

  • Marmur, J. 1961. A procedure for the isolation of deoxyribonucleic acid from microorganisms. - J. Mol. Biol.3: 208–218.

    Google Scholar 

  • Marmur, J., Falkow, S. andMandel, M. 1963. New approaches to bacterial taxonomy. - Ann. Rev. Microbiol.17: 329–372.

    Google Scholar 

  • Sen, M. andSen, S. P. 1965. Interspecific transformation inAzotobacter.- J. Gen. Microbiol.41: 1–6.

    Google Scholar 

  • Sen, S. P. andNandi, P. N. 1961. Inhibition ofRhizobium andAzotobacter by pure antibiotic substances. - Trans. Bose Res. Inst.24: 35–45.

    Google Scholar 

  • Thimann, K. V. 1963. The Life of bacteria. - MacMillan & Co., New York.

    Google Scholar 

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Authors and Affiliations

  1. Microbiology Laboratory, Department of Botany, Kalyani University, W. Bengal, India

    Mira Sen, T. K. Pal & S. P. Sen

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  1. Mira Sen
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  2. T. K. Pal
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  3. S. P. Sen
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Sen, M., Pal, T.K. & Sen, S.P. Intergeneric transformation betweenRhizobium andAzotobacter . Antonie van Leeuwenhoek 35, 533–540 (1969). https://doi.org/10.1007/BF02219171

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  • DOI: https://doi.org/10.1007/BF02219171

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