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Distinction of species in Aureobasidium and related genera by PCR-ribotyping

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Abstract

Taxonomic markers for differentiation of the anamorph genera Aureobasidium, Hormonema and Kabatiella were developed using PCR-ribotyping with the primers 5.8S-R and LR7 for amplification and the restriction enzymes Alul, DdeI, Hhal, MspI and RsaI for digestion. Aureobasidium and Hormonema are optimally differentiated with MspI; DdeI is particularly useful to distinguish aureobasidium, Kabatiella and Selenophoma. Relationships of the anamorph genera Aureobasidium, Hormoneng and Kabatiella with the teleomorph genera Pringsheimia and Dothiora are discussed.

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Author notes

  1. N. A. Yurlova

    Present address: Department of Microbiology, Chemical-Pharmaceutical Institute, 14, Prof. Popov Str., 197376, St. Petersburg, Russia

Authors and Affiliations

  1. Centraalbureau voor Schimmelcultures, P.O. Box 273, 3740 AG, Baarn, The Netherlands

    N. A. Yurlova, J. M. J. Uijthof & G. S. de Hoog

  2. Institute of Molecular Cell Biology, Bio Centrum Amsterdam, Kruislaan 318, 1098 SM, Amsterdam, The Netherlands

    G. S. de Hoog

Authors
  1. N. A. Yurlova
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  2. J. M. J. Uijthof
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  3. G. S. de Hoog
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Yurlova, N.A., Uijthof, J.M.J. & de Hoog, G.S. Distinction of species in Aureobasidium and related genera by PCR-ribotyping. Antonie van Leeuwenhoek 69, 323–329 (1996). https://doi.org/10.1007/BF00399621

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  • DOI: https://doi.org/10.1007/BF00399621

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