Abstract
Although the mechanism of sex-differentiation in crustaceans has yet to be defined, the androgenic gland (AG) is thought to be the exclusive organ that produces the androgenic hormone (AH) which induces male sexual development. This paper presents results of light and transmission electron microscopy and total protein analysis of androgenic glands from three male morphotypes (orange-claw, orange-blue-claw and blue-claw) of the freshwater prawn, Macrobrachium rosenbergii. Highest protein content (76 µg//AG) was found in the blue-claw morphotype as compared to the orange-blue-claw (45 µg/AG) and the orange-claw morphotype (19 µg/AG). Sodiumdodecyl sulfate polyacrylamide gel electrophoresis (SDS-page) analysis of the cell free extract of the AG from the three morphotypes revealed four polypeptides (∼16, ∼18, ∼23 and ∼26 Kd) which quantitatively increase from the sexually immature orange-claw to the sexually mature blue-claw morphotype. The 16 and 18 Kd polypeptides could be the AHs.
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References
Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.
Charniaux-Cotton, H. (1954) Découverte chez un Crustacé Amphipoda (Orchestia gamarella) d'une glande endocrine responsible de la différenciation dos caractéres sexuels primalres et secondaires males. Comptes Rendus Academie des Sciences (Paris) 239, 780–782.
Hasegawa, Y., Hirose, E. and Katakura, Y. (1993) Hormonal control of sexual differentiation and reproduction in Crustacea. American Zoologist 33, 403–411.
Juchault, P., Legrand, J.J. and Maissiat, J. (1984) Present state of knowledge on the chemical nature of the androgenic hormone in higher crustaceans. In: J. Hoffman and M. Porchet (eds.), Biosynthesis, Metabolism and Mode of Action in Invertebrate Hormones. Springer-Verlag, Berlin/Heidelberg, pp. 155–160.
Kuris, M.A., Ra'anan, Z., Sagi, A. and Cohen, D. (1987) Morphotypic differentiation of male Malaysian giant prawn, Macrobrachium rosenbergii. Journal of Crustacean Biology 7, 219–237.
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
Malecha, S.R., Nevin, P.A., Ha, P., Barck, L.E., Lamadrid-Rose, Y., Masuno, S. and Hedgecock, D. (1992) Sex-ratios and sex-determination in progeny from crosses of surgically sex-reversed freshwater prawns, Macrobrachium rosenbergii. Aquaculture 105, 201–218.
Martin, G., Sorokine, O., Moniatte, M. and Vandorsselaer, A. (1998) The androgenic hormone of the crustacean isopod Armadillidium vulgare. Annals of the New York Academy of Sciences 839, 111–117.
Martin, G., Sorokine, O., Moniatte, M., Bulet, P., Hetru, C. and Van Dorsselaer, A. (1999) The structure of a glycosylated protein hormone responsible for sex determination in the isopod, Armadillidium vulgare. European Journal of Biochemistry 262, 727–736.
Nagamine, C., Knight, A.W., Maggenti, A. and Paxman, G. (1980) Effects of androgenic ablation on male primary and secondary sexual characteristics in the Malaysian prawn Macrobrachium rosenbergii (de Man) (Decapoda, Palaemonidae), with first evidence of induced feminization in a non-hermaphrodite decapod. General and Comparative Endocrinology 41, 423–441.
Okuno, A., Hasegawa, Y., Ohira, T., Katakura, Y. and Nagasawa, H. (1999) Characterization and cDNA cloning of androgenic gland hormone of the terrestrial isopod Armadillidium vulgare. Biochemical and Biophysical Research Communications 264, 419–423.
Sagi, A. and Cohen, D. (1990) Growth, maturation and potency of sex-reversed Macrobrachium rosenbergii males. World Aquaculture 21(1), 87–90.
Sagi, A. and Cohen, D. and Milner Y. (1990) Effect of androgenic gland ablation on morphotype differentiation and sexual characteristics of male freshwater prawns, Macrobrachium rosenbergii. General and Comparative Endocrinology 77, 15–22.
Sagi, A., Snir, E. and Khalaila, I. (1997) Sexual differentiation in decapod crustaceans: role of the androgenic gland. Invertebrate Reproduction and Development 31, 55–61.
Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Sun, P.S. (1995) Expression of the molt-inhibiting hormone-like gene in the eyestalk and brain of the white shrimp Penaeus vannamei. Molecular Marine Biology and Biotechnology 4, 262–268.
Taketomi, Y., Murata, M. and Miyawaki, M. (1990) Androgenic gland and secondary sexual characters in the crayfish Procambarus clarkii. Journal of Crustacean Biology 10, 492–497.
Veith, W. and Malecha, S. (1983) Histochemical study of the distribution of lipids, 3ß-and 3ß-hydroxysteroid dehydrogenase in the androgenic gland of the cultured prawn, Macrobrachium rosenbergii (de Man) (Crustacea; Decapoda). South African Journal of Science 79, 84–85.
Weatherby, T.M. (1981) Ultrastructural study of the sinus gland of the crab. Cardisoma carnifex. Cell Tissue Research 220, 293–312.
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Sun, P.S., Weatherby, T.M., Dunlap, M.F. et al. Developmental changes in structure and polypeptide profile of the androgenic gland of the freshwater prawn Macrobrachium rosenbergii. Aquaculture International 8, 327–334 (2000). https://doi.org/10.1023/A:1009205027360
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DOI: https://doi.org/10.1023/A:1009205027360