Summary
The most direct approach to enhancing the volumetric yield of secondary metabolites in plant tissue cultures is to operate the culture under high cell density. In this study, a cell suspension ofAnchusa officinalis was cultivated using a semi-continuous perfusion technique, i.e. batch cultivation with intermittent medium exchange. Using a perfusion medium containing sucrose concentration which was two times that in the normal growth medium, the final cell density and the final product concentration were increased by more than 2-fold compared with a batch culture without medium exchange. The high cell density obtained from the semi-continuous perfusion culture can be explained by the prevention of nutrient depletion, removal of toxic by-products, as well as the control of cell size by virtue of the high sugar medium osmolarity.
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Su, WW., Humphrey, A.E. Production of rosmarinic acid in high density perfusion cultures ofAnchusa officinalis using a high sugar medium. Biotechnol Lett 12, 793–798 (1990). https://doi.org/10.1007/BF01022597
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DOI: https://doi.org/10.1007/BF01022597