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Protein production (β-galactosidase) from a baculovirus vector inSpodoptera frugiperda andTrichpolusia ni cells in suspension culture

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Abstract

Protein production capabilities ofTrichpolusia ni (TN 368) cells andSpodoptera frugiperda (Sf9) cells were compared in GTC100 medium in suspension culture using as a vector a genetically engineeredAutographa californica nuclear polyhedrosis virus. TN 368 produces more β-galactosidase than Sf9, on a per cell basis (2.2×105 and 1.7×105 units/ 106 cells1 respectively). In growth experiments serum-free medium supported a higher maximum Sf9 cell density (4±1×106 cells/ml) than the serum- based media (1.5±5×106 cells/ml in GTC100 and 2±1×106 cells/ml in TNM-FH). However, using a cell density of 5×05 cells/ml, the productivity per cell varied, from a low of 4.5×104 units in EX-CELL-400 medium to a high of 7.6×104 units in TNM-FH. The TN 368 cells were twice a large as Sf9 cells and appeared to be more shear sensitive than Sf9 cells.

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Authors and Affiliations

  1. Chemical engineering Department, Cornell University, 120 Olin hall, 14853, Ithaca, N.Y.

    O. Ogonah & M. L. Shuler

  2. Boyce Thompson Institute for Plant Research, 14853, Ithaca, N.Y.

    R. R. Granados

Authors
  1. O. Ogonah
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  2. M. L. Shuler
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  3. R. R. Granados
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Ogonah, O., Shuler, M.L. & Granados, R.R. Protein production (β-galactosidase) from a baculovirus vector inSpodoptera frugiperda andTrichpolusia ni cells in suspension culture. Biotechnol Lett 13, 265–270 (1991). https://doi.org/10.1007/BF01041482

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  • DOI: https://doi.org/10.1007/BF01041482

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