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Effects of dimethyl sulfoxide on heavy chain monoclonal antibody production from plant cell culture

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Summary

The addition of 4% (v/v) dimethyl sulfoxide (DMSO) increased the total production of heavy chain monoclonal antibody (HC MAb) in suspension culture of genetically modified Nicotiana tabacum by three fold. This may be due to stabilization of HC MAb in the presence of DMSO by forming hydrogen bonds with proton-donor groups on protein molecules.

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Authors and Affiliations

  1. Department of Chemical Engineering, Washington State University, 99164-2710, Pullman, WA, USA

    Michael F. Wahl, Gynheung An & James M. Lee

  2. Institute of Biological Chemistry Washington State University, 99164-2710, Pullman, WA, USA

    Gynheung An

Authors
  1. Michael F. Wahl
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  2. Gynheung An
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  3. James M. Lee
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Wahl, M.F., An, G. & Lee, J.M. Effects of dimethyl sulfoxide on heavy chain monoclonal antibody production from plant cell culture. Biotechnol Lett 17, 463–468 (1995). https://doi.org/10.1007/BF00132011

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  • DOI: https://doi.org/10.1007/BF00132011

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