Abstract
Using the modified yeast expression vectors that contained phosphoglycerate kinase or chelatin promoter with the adenine (A) base at –3 position from the ATG coding sequences and the leu-2 gene, recombinant human inter-leukin-6 ( rhIL-6) was produced as a b-galactosidase ( lacZ) fusion protein in Saccharomyces cerevisiae. Expression level of the IL-6-lacZ was 12 times higher than that from the unmodified wild type plasmids and IL-6 activity was 9–9.8 x 10 5 unit/mg yeast total protein. Thus these modified yeast vectors are useful for high expression of foreign genes in yeast
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Chung, KS., Kang, HS., Kim, KW. et al. Expression of recombinant human interleukin 6 ( rhIL6) in Saccharomyces cerevisiae by the modified phosphoglycerate kinase and chelatin promoter. Biotechnology Letters 19, 1169–1173 (1997). https://doi.org/10.1023/A:1018421517371
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DOI: https://doi.org/10.1023/A:1018421517371