Abstract
α-1,2-Mannosidase from Trichoderma reesei was used to modify the N-linked glycosylation pathway of the methylotrophic yeast Pichia pastoris. Expression of foreign influenza glycoproteins with more extensively processed N-linked oligosaccharides was observed when α-1,2-mannosidase was secreted in the culture medium. However, intracellular removal of mannose residues may stimulate mannosyltransferase activity and leads to hyperglycosylation. [3H]Mannose suicide selection or high concentrations of orthovanadate, commonly used to isolate glycosylation mutants of Saccharomyces cerevisiae, had no profound effect on Pichia pastoris. © Rapid Science Ltd. 1998
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Martinet, W., Maras, M., Saelens, X. et al. Modification of the protein glycosylation pathway in the methylotrophic yeast Pichia pastoris. Biotechnology Letters 20, 1171–1177 (1998). https://doi.org/10.1023/A:1005340806821
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DOI: https://doi.org/10.1023/A:1005340806821