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Enzymatic Synthesis of N-formyl-L-aspartyl-L-phenylalanine Methyl Ester (Aspartame Precursor) Utilizing an Extractive Reaction in Aqueous/Organic Biphasic Medium

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Abstract

N-Formyl-L-aspartyl-L-phenylalanine methyl ester (N-formyl aspartame, F-AspPheOMe) was synthesized enzymatically utilizing an extractive reaction in an aqueous/organic biphasic system. The N-formyl aspartame yield in a pure aqueous monophasic system was, in general, ca. 3% , however, it was over 80 % in a water/1-butanol biphasic system using a simultaneously extractive operation of an enzymatic reaction in an aqueous phase and a product separation from an aqueous to an organic phases.

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Authors and Affiliations

  1. Department of Chemical Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo, 169-8555, Japan

    Yoshihiko Murakami, Tatsunori Yoshida & Akira Hirata

Authors
  1. Yoshihiko Murakami
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  2. Tatsunori Yoshida
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  3. Akira Hirata
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Murakami, Y., Yoshida, T. & Hirata, A. Enzymatic Synthesis of N-formyl-L-aspartyl-L-phenylalanine Methyl Ester (Aspartame Precursor) Utilizing an Extractive Reaction in Aqueous/Organic Biphasic Medium. Biotechnology Letters 20, 767–769 (1998). https://doi.org/10.1023/B:BILE.0000015919.00464.b0

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  • DOI: https://doi.org/10.1023/B:BILE.0000015919.00464.b0

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