Abstract
Genetic transformation of a biological oxygen demand (BOD)-sensing yeast, Trichosporon cutaneum IFO 10466 was carried out using a plasmid shuttle vector, pAN 7–1, between Escherichia coli and Aspergillus niger and Aspergillus nidulans. The hygromycin B-resistant transformants were obtained with the plasmid by using the spheroplasts. The transformation frequency was up to 70 colonies per μg of plasmid DNA. Southern-blot analysis and transformation test of E. coli using DNA extracts from hygromycin B-resistant transformants of IFO 10466 indicated that pAN 7–1 existed independently in the cells without integration into the chromosome. These results suggest that pAN 7–1 has potential availability for introduction and expression of the external gene such as a bioluminescence gene, lux or luc, to create a luminous yeast for BOD sensing. © Rapid Science Ltd. 1998
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barnett, JA, Payne, RW and Yarrow, D (1990). Descriptions of the species, 562 Trichosporon beigelii.In: Yeasts - Characterization and Identification, 2 nd. eds pp 664-665, Cambridge: Cambridge University Press
Birnboim, HC (1983). Methods in Enzymol 100: 243-255
Glumoff, V, Käppeli, O, Fiechter, A and Reiser, J (1989). Gene 84: 311-318
Gritz, L, and Davies, J (1983). Gene 25: 179-188
Guého, E, Kurtzman, CP and Peterson, SW (1989). Syst Appl Microbiol 12: 230-236
Guého, E, Tredick, J and Phaff, HJ (1984). Antonie van Leeuwenhoek 50: 17-32
Hamlyn, PF, Bradshaw, RE, Mellon, FM., Santiago, CM, Wilson, JM and Peberdy, JF (1981). Enzyme Microb Technol 3: 321-325
Hara, N, Tubota, Y, Saito, K and Suto, T (1989). J Gen Appl Microbiol 35: 1-10
Hikuma, M, Suzuki, H, Yasuda, T, Karube, I and Suzuki, S (1979). European J Appl Microbiol Biotechnol 8: 289-297.
Hikuma, M and Yasuda, T (1988). Methods in Enzymol 137: 124-131
Hoffman, CS and Winston, F (1987). Gene 57: 267-272
Hyun, C-K, Tamiya, E, Takeuchi, T, Karube, I and Inoue, N (1993). Biotechnol Bioeng 41: 1107-1111
Japanese Industrial Standard Committee (1991). JIS K 3602, Tokyo, Japan, pp 1-14
Karube, I, Matsunaga, T, Mitsuda, S and Suzuki, S (1977a). Biotechnol Bioeng 14: 1533-1547
Karube, I, Mitsuda, S, Matsunaga, T and Suzuki, S (1977b). J Ferment Technol 55: 243-248
Mörtberg, M and Neujahr, HY (1986). J Bacteriol 168: 734-738
Punt, PJ, Oliver, RP, Dingemanse, MA, Pouwels, PH and van den Hondel, CAMJJ (1987). Gene 56: 117-124
Sambrook, J, Fritsch, EF, and Maniatis, T (1989) Molecular Cloning, A Laboratory Manual, Cold Spring Harbor, New York: Cold Spring Harbor Press
Southern, EM (1975). J Mol Biol 98: 503-517
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sakaguchi, T., Amari, S., Nagashio, N. et al. Genetic transformation of Trichosporon cutaneum with a plasmid, pAN 7–1, from filamentous fungi. Biotechnology Letters 20, 851–855 (1998). https://doi.org/10.1023/A:1005311509043
Issue Date:
DOI: https://doi.org/10.1023/A:1005311509043