Abstract
α-Chymotrypsin was covalently modified with cellobiose by chemical means. After adsorption on to a porous polyamide support, both the native and the glycosylated immobilized derivatives were used to synthesize a kyotorphin derivative (N-benzoyl-l-tyrosyl-l-argininamide) in acetonitrile/water. Glycosylated chymotrypsin gave a 125% increase in product formation (750 nmol mg−1 catalyst in 3 h) at 60% (v/v) acetonitrile/water. Maximal protective effect of this glycosylation process was at 70% (v/v) acetonitrile/water, at which concentration the half-life of the glycosylated enzyme was 20-times longer than that of the native form (52 min and 2.8 min, respectively).
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Levitsky, V., Lozano, P. & Iborra, J. Glycosylated α-chymotrypsin as a catalyst for kyotorphin synthesis in water-organic media. Biotechnology Letters 21, 595–599 (1999). https://doi.org/10.1023/A:1005503429715
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DOI: https://doi.org/10.1023/A:1005503429715