Abstract
As environmental compatible emulsifiers, various polysaccharides were investigated and de-emulsification methods were studied using filtration, direct current (D.C.) field, and also Nocardia amarae. 0.01% (v/v) of alginic acid, arabic gum, chitosan, and curdlan showed more than about 2 h of emulsion stability in the half-life of emulsion layer by a homogenizer and showed about twice synergic effects with other polysaccharides in emulsification activity at the ratio of 9:1 (v/v). De-emulsification by 110V D.C. field took about 1 h for the separation of oils from 1 l of oil-in-water emulsion in case of 0.01% arabic gum. Oil-water separator was designed using non-woven fabrics in filtration system and Nocardia amarae grown in n-hexadecane or kerosene was immobilized in the non-woven fabrics.
Similar content being viewed by others
References
Cairns WL, Cooper DG, Zajic JE, Wood JM, Kosaric N (1982) Characterization of Nocardia amarae as a potent biological coalescing agent of water-oil emulsions. Appl. Environ. Microbiol. 43: 362–366.
Gray NCC, Stewart AL, Cairns WL, Kosaric N (1984) Bacteriainduced de-emulsification of oil-in-water petroleum emulsions. Biotechnol. Lett. 6: 419–424.
Hano T, Ohtake T, Takagi K (1988) De-emulsification kinetics of water-in-oil emulsion in an alternating current electric field. J. Chem. Eng. Japan 21: 345–351.
Kosaric N, Cairns WL, Gray NCC (1987) Biosurfactants and Biotechnology. New York: Marcel Dekker.
Stewart AL, Gray NCC, Cairns WL, Korsaric N (1983) Bacteriainduced de-emulsification of water-in-oil emulsions. Biotechnol. Lett. 5: 725–730.
Taylor SE (1996) Theory and practice of electrically-enhanced phase separation of water-in-oil emulsion. Trans IChemE 74: 526–540.
Wang SS, Lee CJ, Chan CC (1994) De-emulsification of water-inoil emulsions by use of a high voltage alternating current field. Sep. Sci. Tech. 29: 159–170.
Yalpani M (1988) Polysaccharides: Synthesis, Modifications and Structure/Property Relations. New York: Elsevier.
Zuckerberg A, Diver A, Perri D, Gutnick DL, Rosenberg E (1979) Emulsifier Acinetobacter RAG-1: chemical and physical properties. Appl. Environ. Microbiol. 37: 414–420.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lee, JC., Lee, KY. Emulsification using environmental compatible emulsifiers and de-emulsification using D.C. field and immobilized Nocardia amarae. Biotechnology Letters 22, 1157–1163 (2000). https://doi.org/10.1023/A:1005601525934
Issue Date:
DOI: https://doi.org/10.1023/A:1005601525934