Abstract
The new fructosyltransferase (FTase) from Bacillus maceransEG-6 showed a broad acceptor specificity, and resulted in the formation of fructosylxyloside (FX) with d-xylose being the most effective acceptor. The optimal FTase concentration for FX production was 0.6 unit per g sucrose, which gave the highest transfer ratio, 83%, of fructosyl moiety from sucrose to d-xylose. Maximum yield of FX was 114 g l−1with 200 g sucrose l−1and 300 g d-xylose l−1.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Fujita K, Hara K, Hashimoto H, Kitahata S (1989) Studies on production of xylosylfructoside by levansucrase. Nippon Shokuhin Kogyo Gakkaishi 36: 910-915.
Fujita K, Hara K, Hashimoto H, Kitahata S (1990) Transfructosylation catalyzed by β-fructofuranosidase I from Arthrobactersp. K-1. Agric. Biol. Chem. 54: 2655-2661.
Hidaka H, Hirayama M, Sumi N (1988) A fructooligosaccharidesproducing enzyme from Aspergillus nigerATCC 20611. Agric. Biol. Chem. 52: 1181-1187.
Ito Y, Shimura S (1988) The synthesis of xylosucrose by β-fructofuranosidase. Bioindustry 15: 269-275.
Kim BW, Choi JW, Yun JW (1998) Selective production of GF4-fructooligosaccharide from sucrose by a new transfructosylating enzyme. Biotechnol. Lett. 20: 1031-1034.
Kitahata S, Suetake S, Okada S (1986) Purification and transfructosylation reaction of β-fructofuranosidase from Penicillumsp. K25. Nippon Shokuhin Kogyo Gakkaishi 33: 826-830.
Monsan P, Paul K (1995) Enzymatic synthesis of oligosaccharides. FEMS Microbiol. Rev. 16: 187-192.
Nisizawa T, Takeuchi K, Imai S (1986) Difference in mode of inhibition between α-D-xylosyl β-D-fructoside and α-isomaltosyl β-D-fructoside in the synthesis of glucan by Streptococcus mutansD-glucosyltransferase. Carbohydr. Res. 147: 135-144.
Ohtsuka K, Hino S, Fukushima T, Ozawa O, Kanematsu T, Uchida T (1992) Characterization of levansucrase from Rahnella aquatilisJCM-1683. Biosci. Biotechnol. Biochem. 56: 1373-1377.
Takeda H, Kinoshita S (1995) Production of fructosylxylosides by Scopulariopsis brevicaulissp. J. Ferment. Bioeng. 79: 242-246.
Yun JW (1996) Fructooligosaccharides-occurrence, preparation, and application. Enz. Microbiol. Technol. 19: 107-117.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nam, SW., Yun, HJ., Ahn, JH. et al. Production of fructosylxyloside by the new fructosyltransferase from Bacillus macerans EG-6. Biotechnology Letters 22, 1243–1246 (2000). https://doi.org/10.1023/A:1005665400590
Issue Date:
DOI: https://doi.org/10.1023/A:1005665400590